PMID- 6782109
OWN - NLM
STAT- MEDLINE
DCOM- 19810513
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 88
IP  - 1
DP  - 1981 Jan
TI  - Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and 
      membrane-bound polysomes.
PG  - 29-36
AB  - We have analyzed the sequence complexity, frequency distribution, and template 
      activity of free (F) and membrane-bound (MB) polysomal mRNA populations of MOPC 
      21 (P3K) mouse myeloma cells. Using the technique of mRNA-cDNA hybridization, we 
      find that F poly(A)+ RNA, which represent 60% of total polysomal mRNA, consists 
      of approximately 8,000 different mRNA sequences distributed in three abundance 
      classes, while MB poly(A)+ RNA (20% of total polysomal mRNA) includes only 230 
      mRNA species and almost completely lacks very infrequent mRNA species. 
      Cross-hybridization indicates that MB mRNA sequences are also present in F mRNA, 
      but in reduced concentrations. Translation of F and MB RNA fractions in a 
      messenger-dependent reticulocyte lysate indicates that essentially all MB RNA 
      contains poly(A), whereas 25% of F mRNA lacks poly(A). Furthermore, the use of a 
      cDNA highly specific for the immunoglobulin light (Ig L) chain mRNA allows the 
      determination of the subcellular content of this message. Ig L mRNA, representing 
      approximately 5% of total polysomal poly(A)+ RNA, is one of the most abundant MB 
      mRNAs. 90% of Ig L mRNA is found in MB polysomes and 10% in F polysomes.
FAU - Mechler, B
AU  - Mechler B
FAU - Rabbitts, T H
AU  - Rabbitts TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Endoplasmic Reticulum/*metabolism
MH  - Immunoglobulin Light Chains
MH  - Mice
MH  - Multiple Myeloma
MH  - Nucleic Acid Hybridization
MH  - Poly A/analysis
MH  - Polyribosomes/*analysis/metabolism
MH  - Protein Biosynthesis
MH  - RNA, Messenger/*analysis/genetics
PMC - PMC2111720
EDAT- 1981/01/01 00:00
MHDA- 1981/01/01 00:01
PMCR- 1981/07/01
CRDT- 1981/01/01 00:00
PHST- 1981/01/01 00:00 [pubmed]
PHST- 1981/01/01 00:01 [medline]
PHST- 1981/01/01 00:00 [entrez]
PHST- 1981/07/01 00:00 [pmc-release]
AID - 81142490 [pii]
AID - 10.1083/jcb.88.1.29 [doi]
PST - ppublish
SO  - J Cell Biol. 1981 Jan;88(1):29-36. doi: 10.1083/jcb.88.1.29.