PMID- 6787034
OWN - NLM
STAT- MEDLINE
DCOM- 19810820
LR  - 20190511
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 89
IP  - 2
DP  - 1981 Feb
TI  - Presence of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis 
      vegetative cells.
PG  - 511-6
AB  - Extracts of Bacillus subtilis vegetative cells with 2 M formic acid contained a 
      large amount of a hyperphosphorylated nucleotide ("spot 4" nucleotide). The 
      compound always comigrated with authentic guanosine 5'-diphosphate 3'-diphosphate 
      (ppGpp) on two-dimensional polyethyleneimine (PEI)-cellulose thin-layer 
      chromatography performed with three different solvent systems. Furthermore, all 
      dephosphorylated 32P-labeled derivatives from the "spot 4" nucleotide comigrated 
      on a one-dimensional PEI-cellulose plate with those from authentic ppGpp present 
      in the same reaction mixture, when the compounds were hydrolyzed with snake venom 
      phosphodiesterase or alkali. The level of the "spot 4" nucleotide (ppGpp) in the 
      cell extracts was 0.14 nmol P/A660, corresponding to about one-third of the 
      guanosine 5'-triphosphate (GTP) level and about 10% of the adenosine 
      5'-triphosphate (ATP) level. These results indicate that a "magic spot" 
      nucleotide, ppGpp, is present at a high level in B. subtilis cells vegetatively 
      growing in mNSMP.
FAU - Ikehara, K
AU  - Ikehara K
FAU - Ando, H
AU  - Ando H
FAU - Takada, Y
AU  - Takada Y
FAU - Sugae, K
AU  - Sugae K
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Guanine Nucleotides)
RN  - 33503-72-9 (Guanosine Tetraphosphate)
SB  - IM
MH  - Bacillus subtilis/cytology/*metabolism
MH  - Chromatography, Thin Layer
MH  - Guanine Nucleotides/*metabolism
MH  - Guanosine Tetraphosphate/*metabolism
MH  - Hydrolysis
EDAT- 1981/02/01 00:00
MHDA- 1981/02/01 00:01
CRDT- 1981/02/01 00:00
PHST- 1981/02/01 00:00 [pubmed]
PHST- 1981/02/01 00:01 [medline]
PHST- 1981/02/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a133226 [doi]
PST - ppublish
SO  - J Biochem. 1981 Feb;89(2):511-6. doi: 10.1093/oxfordjournals.jbchem.a133226.