PMID- 679183
OWN - NLM
STAT- MEDLINE
DCOM- 19781027
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 38
IP  - 9
DP  - 1978 Sep
TI  - Lactate dehydrogenase in estrogen-responsive human breast cancer cells.
PG  - 2773-6
AB  - Lactate dehydrogenase activity (LDH) was measured in the MCF-7 human breast 
      cancer cell line derived at the Michigan Cancer Foundation from a patient with 
      metastatic breast adenocarcinoma. LDH was found in the 46,000 X g supernatant of 
      cell lysates, but not in the culture medium. Only the fifth isozyme (LDH-5) could 
      be demonstrated by cellulose acetate electrophoresis and relative heat 
      inactivation studies. When endogenous steroids were removed from the medium, 
      addition of estrogen to the growth medium for several days elevated LDH 2-fold 
      above controls; LDH was not altered when MCF-7 cells were treated with 
      progesterone, hydrocortisone, prolactin, insulin, or triiodothyronine. A 
      physiological concentration (0.1 nM) of 17beta-estradiol was sufficient to 
      produce a maximal LDH increase. There were no qualitative isozyme changes in 
      response to estrogen. LDH activity may therefore be a useful marker protein for 
      studying hormone action in the MCF-7 human breast cancer cell line.
FAU - Burke, R E
AU  - Burke RE
FAU - Harris, S C
AU  - Harris SC
FAU - McGuire, W L
AU  - McGuire WL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Isoenzymes)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Adenocarcinoma/drug therapy/*enzymology
MH  - Breast Neoplasms/drug therapy/*enzymology
MH  - Cell Line
MH  - Enzyme Activation/drug effects
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Humans
MH  - Isoenzymes
MH  - L-Lactate Dehydrogenase/*metabolism
MH  - Neoplasms, Experimental/enzymology
EDAT- 1978/09/01 00:00
MHDA- 1978/09/01 00:01
CRDT- 1978/09/01 00:00
PHST- 1978/09/01 00:00 [pubmed]
PHST- 1978/09/01 00:01 [medline]
PHST- 1978/09/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1978 Sep;38(9):2773-6.