PMID- 6800203
OWN - NLM
STAT- MEDLINE
DCOM- 19820422
LR  - 20180216
IS  - 0001-5792 (Print)
IS  - 0001-5792 (Linking)
VI  - 67
IP  - 1
DP  - 1982
TI  - Interference of serum tonicity with the measurement of red cell mean corpuscular 
      volume.
PG  - 62-6
AB  - When red cell mean corpuscular volume (MCV) is determined by use of the Coulter 
      counter, the blood sample is first diluted in a solution with fixed osmolality 
      (Isoton). Therefore we studied the interference of blood osmolality with MCV 
      measured by this method. In 14 patients with hyposmolality, the correction of 
      osmolality was accompanied by an increase in MCV of 4.7% (p less than 0.001) and 
      a decrease in mean corpuscular hemoglobin concentration (MCHC) of 4.8% (p less 
      than 0.001). In 9 patients with hyperosmolality the decrease in MCV was of 5% (p 
      less than 0.001) and the rise in MCHC of 4% (p less than 0.001) after osmolality 
      correction. Before correction of hyposmolality, 1 patient had false microcytosis 
      and 3 had masked macrocytosis. In the hypertonic group 3 patients has initially 
      false macrocytosis. Red blood cells (RBC) from hypotonic patients probably shrink 
      when they are acutely placed in the Isoton which is a hypertonic solution (330 
      mosm/kg H2O). Conversely RBC from patients with severe hyperosmolality swell in 
      the same conditions. The patients osmolality must be considered to interpret the 
      MCV measured by the Coulter counter correctly, as a 10-mosm/kg H2O change in 
      serum osmolality is responsible for an artefactual change in MCV of 1 fl.
FAU - Decaux, G
AU  - Decaux G
FAU - Efira, A
AU  - Efira A
FAU - Dhaene, M
AU  - Dhaene M
FAU - Unger, J
AU  - Unger J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Acta Haematol
JT  - Acta haematologica
JID - 0141053
SB  - IM
MH  - *Blood Physiological Phenomena
MH  - *Erythrocyte Indices
MH  - Erythrocytes/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Osmolar Concentration
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1159/000207026 [doi]
PST - ppublish
SO  - Acta Haematol. 1982;67(1):62-6. doi: 10.1159/000207026.