PMID- 6814337
OWN - NLM
STAT- MEDLINE
DCOM- 19821216
LR  - 20170214
IS  - 0004-5632 (Print)
IS  - 0004-5632 (Linking)
VI  - 19
IP  - Pt 5
DP  - 1982 Sep
TI  - Major factors limiting sensitivity of sandwich enzyme immunoassay for ferritin, 
      immunoglobulin E, and thyroid-stimulating hormone.
PG  - 379-84
AB  - By using IgG-coated polystyrene balls and beta-D-galactosidase-labelled Fab', 
      sandwich enzyme immunoassays for human ferritin, immunoglobulin E (IgE), and 
      thyroid-stimulating hormone (TSH) were developed, and their sensitivities were 
      shown to be largely limited by the purity, binding efficiency, and amount of 
      beta-D-galactosidase-labelled Fab' used. (1) Their sensitivities were enhanced 10 
      to 50-fold by using affinity-purified Fab' labelled with beta-D-galactosidase. 
      (2) Their sensitivities depended upon the efficiency of specific binding of the 
      labelled Fab' to antigens adsorbed on antibody IgG-coated polystyrene balls. (3) 
      Their sensitivities were improved by using the minimal amount of labelled Fab' 
      that gave a reliable calibration curve. Under optimal conditions the 
      sensitivities obtained for ferritin, IgE, and TSH were 0.09 pg (0.2 amol), 0.48 
      pg (2.4 amol, 0.2 mIU), and 3.2 millimicronU (5.7 amol) per tube, respectively.
FAU - Ishikawa, E
AU  - Ishikawa E
FAU - Imagawa, M
AU  - Imagawa M
FAU - Yoshitake, S
AU  - Yoshitake S
FAU - Niitsu, Y
AU  - Niitsu Y
FAU - Urushizaki, I
AU  - Urushizaki I
FAU - Inada, M
AU  - Inada M
FAU - Imura, H
AU  - Imura H
FAU - Kanazawa, R
AU  - Kanazawa R
FAU - Tachibana, S
AU  - Tachibana S
FAU - Nakazawa, N
AU  - Nakazawa N
FAU - Ogawa, H
AU  - Ogawa H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ann Clin Biochem
JT  - Annals of clinical biochemistry
JID - 0324055
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9002-71-5 (Thyrotropin)
RN  - 9007-73-2 (Ferritins)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Ferritins/*analysis
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin E/*analysis
MH  - Immunoglobulin Fab Fragments
MH  - Thyrotropin/*analysis
MH  - beta-Galactosidase
EDAT- 1982/09/01 00:00
MHDA- 1982/09/01 00:01
CRDT- 1982/09/01 00:00
PHST- 1982/09/01 00:00 [pubmed]
PHST- 1982/09/01 00:01 [medline]
PHST- 1982/09/01 00:00 [entrez]
AID - 10.1177/000456328201900511 [doi]
PST - ppublish
SO  - Ann Clin Biochem. 1982 Sep;19(Pt 5):379-84. doi: 10.1177/000456328201900511.