PMID- 6817747
OWN - NLM
STAT- MEDLINE
DCOM- 19830225
LR  - 20221207
IS  - 0006-2928 (Print)
IS  - 0006-2928 (Linking)
VI  - 20
IP  - 9-10
DP  - 1982 Oct
TI  - Chemical and enzymological characterization of an Indonesian variant of human 
      erythrocyte carbonic anhydrase II, CAII Jogjakarta (17 Lys leads to Glu).
PG  - 979-1000
AB  - A new variant of human erythrocyte carbonic anhydrase II (CAII) was discovered in 
      a single heterozygous individual during routine screening of blood samples from 
      the island of Java in Indonesia. The normal and variant components of the 
      heterozygous CAII mixture were resolved by isoelectric focusing following 
      purification by a specific affinity matrix. Specific esterase activities and 
      Michaelis-Menten constants were identical. Only very small differences were noted 
      with respect to inhibition by acetazolamide and chloride. Double diffusion 
      analysis showed the immunological identify of the normal and variant enzymes. The 
      variant CAII was considerably less heat stable than the normal enzyme. The 
      variant was slightly more stable than the normal enzyme upon dialysis against the 
      zinc chelator dipicolinic acid (PDCA), indicating a tighter binding of zinc than 
      the normal enzyme. Analysis of tryptic peptides from the normal and variant 
      enzymes indicated that, in the variant, lysine at position 17 from the N terminus 
      had changed to glutamic acid. The differences in physiochemical properties 
      observed for the normal and variant enzyme are discussed in relation to the 
      possible effects of this substitution on the structure of the CAII molecule.
FAU - Jones, G L
AU  - Jones GL
FAU - Sofro, A S
AU  - Sofro AS
FAU - Shaw, D C
AU  - Shaw DC
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
RN  - 0 (Carbonic Anhydrase Inhibitors)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Amino Acid Sequence
MH  - Asian People
MH  - Carbonic Anhydrase Inhibitors
MH  - Carbonic Anhydrases/blood/*genetics/immunology
MH  - Erythrocytes/*enzymology
MH  - Hot Temperature
MH  - Humans
MH  - Indonesia
MH  - Isoelectric Point
MH  - Kinetics
MH  - Male
MH  - Molecular Weight
MH  - Zinc/blood
EDAT- 1982/10/01 00:00
MHDA- 1982/10/01 00:01
CRDT- 1982/10/01 00:00
PHST- 1982/10/01 00:00 [pubmed]
PHST- 1982/10/01 00:01 [medline]
PHST- 1982/10/01 00:00 [entrez]
AID - 10.1007/BF00484072 [doi]
PST - ppublish
SO  - Biochem Genet. 1982 Oct;20(9-10):979-1000. doi: 10.1007/BF00484072.