PMID- 6829769
OWN - NLM
STAT- MEDLINE
DCOM- 19830415
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 244
IP  - 3
DP  - 1983 Mar
TI  - Migrating action-potential complexes in vitro in cholera-exposed rabbit ileum.
PG  - G291-4
AB  - The objective of this study was to determine whether cholera-exposed rabbit ileum 
      exhibits altered myoelectric activity in vitro, without central nervous system 
      connections. Whole-cell lysate of Vibrio cholerae, 100 mg in 1 ml saline, was 
      injected into the jejunum of New Zealand White rabbits. Segments of ileum were 
      removed at 12 and 24 h after inoculation and studied in vitro using myoelectric 
      recording techniques. Propagating ring contractions were visualized and 
      corresponded to intense action-potential activity that propagated over 
      consecutive electrode sites. This altered myoelectric activity was similar to the 
      previously described migrating action-potential complex (MAPC) in vivo after 
      infection of rabbit ileum with live V. cholerae, its wholecell lysate, or the 
      purified enterotoxin choleragen, with one exception. All MAPC activity propagated 
      aborally in the in vivo-infected loops; in contrast 26% of the MAPCs propagated 
      retrograde in the in vitro loops. Control segments were injected with saline, and 
      no in vitro MAPCs were observed. Thus, the MAPC stimulated by cholera toxin may 
      be maintained by the enteric nervous system of the gut wall. Although a role for 
      extrinsic nerves is not excluded, our observations suggest that the small 
      intestine may work autonomously, independent of the central nervous system.
FAU - Koch, K L
AU  - Koch KL
FAU - Martin, J L
AU  - Martin JL
FAU - Mathias, J R
AU  - Mathias JR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Cholera/*physiopathology
MH  - Cholera Toxin/pharmacology
MH  - Ileum/drug effects/*physiopathology
MH  - Male
MH  - Muscle, Smooth/drug effects/*physiopathology
MH  - Rabbits
MH  - Vibrio cholerae
OID - NASA: 83150225
EDAT- 1983/03/01 00:00
MHDA- 1983/03/01 00:01
CRDT- 1983/03/01 00:00
PHST- 1983/03/01 00:00 [pubmed]
PHST- 1983/03/01 00:01 [medline]
PHST- 1983/03/01 00:00 [entrez]
AID - 10.1152/ajpgi.1983.244.3.G291 [doi]
PST - ppublish
SO  - Am J Physiol. 1983 Mar;244(3):G291-4. doi: 10.1152/ajpgi.1983.244.3.G291.