PMID- 6832219
OWN - NLM
STAT- MEDLINE
DCOM- 19830505
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 143
IP  - 2
DP  - 1983 Feb
TI  - Acidic vesicles and the uptake of amines by sea urchin eggs.
PG  - 319-25
AB  - The radioactive amine [14C]methylamine is accumulated to a great extent by eggs, 
      with kinetics that are dependent upon temperature (Q10 = 5) and sensitive to 
      metabolic inhibitors. Efflux of [14C]methylamine from eggs (preloaded with tracer 
      concentrations) is increased immediately after fertilization or NH4Cl activation. 
      Fluorescent amines (9-aminoacridine (9AA) and acridine orange (AO)) are 
      concentrated in small intracellular compartments, presumably vesicles. The 
      possible role of these vesicles in the accumulation of amines by sea urchin eggs 
      and the activation of the metabolism that ensues is discussed.
FAU - Christen, R
AU  - Christen R
LA  - eng
GR  - GM 23910/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Aminoacridines)
RN  - 0 (Methylamines)
RN  - 01Q9PC255D (Ammonium Chloride)
RN  - 78OY3Z0P7Z (Aminacrine)
RN  - BSF23SJ79E (methylamine)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Aminacrine/*metabolism
MH  - Aminoacridines/*metabolism
MH  - Ammonium Chloride/pharmacology
MH  - Animals
MH  - Biological Transport
MH  - Cell Compartmentation
MH  - Female
MH  - Fertilization
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Methylamines/*metabolism
MH  - Organoids/metabolism
MH  - Ovum/*metabolism
MH  - Sea Urchins
MH  - Temperature
EDAT- 1983/02/01 00:00
MHDA- 1983/02/01 00:01
CRDT- 1983/02/01 00:00
PHST- 1983/02/01 00:00 [pubmed]
PHST- 1983/02/01 00:01 [medline]
PHST- 1983/02/01 00:00 [entrez]
AID - 10.1016/0014-4827(83)90057-5 [doi]
PST - ppublish
SO  - Exp Cell Res. 1983 Feb;143(2):319-25. doi: 10.1016/0014-4827(83)90057-5.