PMID- 6832224
OWN - NLM
STAT- MEDLINE
DCOM- 19830505
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 143
IP  - 2
DP  - 1983 Feb
TI  - Effects of 3-aminobenzamide on DNA synthesis and cell cycle progression in 
      Chinese hamster ovary cells.
PG  - 377-82
AB  - 3-Aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) polymerase, is a potent 
      inducer of sister chromatid exchanges (SCEs). Because of the possible relation 
      between SCEs and DNA synthesis, the effects of 3AB on DNA synthesis and cell 
      cycle progression in Chinese hamster ovary (CHO) cells were examined. Unlike all 
      other SCE-inducing agents whose effects on DNA synthesis have been studied, short 
      term exposures (30-120 min) of 3AB did not inhibit the overall rate of DNA 
      synthesis and this result was independent of the amount of bromodeoxyuridine 
      (BrdU) in the DNA. Longer exposure times (greater than 24 h) did result in an 
      extended S phase, but this was not due to an effect on the rate of DNA chain 
      elongation. 3AB also delayed the entry of cells into S phase. The overall cell 
      cycle delay was dose dependent, approaching 9 h after a 54 h exposure to 10 mM 
      3AB. Earlier reports that 3AB is neither mutagenic nor cytotoxic were confirmed. 
      Thus 3AB acts to increase SCE frequency by a mechanism distinct from that which 
      causes cytotoxicity and mutagenicity, and does not involve any inhibition in the 
      rate of DNA chain growth.
FAU - Schwartz, J L
AU  - Schwartz JL
FAU - Morgan, W F
AU  - Morgan WF
FAU - Kapp, L N
AU  - Kapp LN
FAU - Wolff, S
AU  - Wolff S
LA  - eng
GR  - CA 06905/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Benzamides)
RN  - 8J365YF1YH (3-aminobenzamide)
RN  - 9007-49-2 (DNA)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Benzamides/*pharmacology
MH  - Cell Line
MH  - Cricetinae
MH  - DNA/*biosynthesis
MH  - Female
MH  - Interphase/*drug effects
MH  - Kinetics
MH  - Mutation/drug effects
MH  - Ovary
MH  - Sister Chromatid Exchange/drug effects
MH  - Thymidine/metabolism
EDAT- 1983/02/01 00:00
MHDA- 1983/02/01 00:01
CRDT- 1983/02/01 00:00
PHST- 1983/02/01 00:00 [pubmed]
PHST- 1983/02/01 00:01 [medline]
PHST- 1983/02/01 00:00 [entrez]
AID - 10.1016/0014-4827(83)90064-2 [doi]
PST - ppublish
SO  - Exp Cell Res. 1983 Feb;143(2):377-82. doi: 10.1016/0014-4827(83)90064-2.