PMID- 6839883
OWN - NLM
STAT- MEDLINE
DCOM- 19830610
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 3
IP  - 5
DP  - 1983 Mar
TI  - Distinction between 5-bromodeoxyuridine labeled and unlabeled mitotic cells by 
      flow cytometry.
PG  - 345-8
AB  - Exponentially growing Friend leukemia cells are exposed to 5-bromodeoxyuridine 
      (BrdUrd) for the time period equivalent to one generation. After fixation and 
      incubation with RNase, DNA in situ is partially denatured by acid and the cells 
      are stained with acridine orange (AO). Staining with AO under these conditions 
      reveals the extent of denatured versus double stranded DNA and enables one to 
      distinguish mitotic from interphase cells. It is observed that all BrdUrd treated 
      cells, regardless of cell cycle phase, have decreased both green and red 
      fluorescence. This finding suggests that BrdUrd interacts with AO not only in the 
      complexes of the dye with double stranded DNA, but also with the single stranded 
      biopolymer. The BrdUrd-attributed suppression of cell fluorescence is large 
      enough to separate totally BrdUrd labeled from unlabeled mitotic populations. The 
      combination of these two flow cytometric techniques, one which allows 
      discrimination between interphase and metaphase cells and the other which allows 
      further subdivision of metaphase cells into those which have incorporated BrdUrd 
      and those which have not, provides an alternative to the autoradiographic 
      procedure necessary for obtaining the fraction of labeled mitoses.
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Traganos, F
AU  - Traganos F
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
GR  - CA14134/CA/NCI NIH HHS/United States
GR  - CA23296/CA/NCI NIH HHS/United States
GR  - CA28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - F30N4O6XVV (Acridine Orange)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Bromodeoxyuridine/*metabolism
MH  - *Flow Cytometry
MH  - Friend murine leukemia virus
MH  - Leukemia, Experimental/pathology
MH  - *Mitosis
EDAT- 1983/03/01 00:00
MHDA- 1983/03/01 00:01
CRDT- 1983/03/01 00:00
PHST- 1983/03/01 00:00 [pubmed]
PHST- 1983/03/01 00:01 [medline]
PHST- 1983/03/01 00:00 [entrez]
AID - 10.1002/cyto.990030507 [doi]
PST - ppublish
SO  - Cytometry. 1983 Mar;3(5):345-8. doi: 10.1002/cyto.990030507.