PMID- 6842383
OWN - NLM
STAT- MEDLINE
DCOM- 19830610
LR  - 20190711
IS  - 0022-3549 (Print)
IS  - 0022-3549 (Linking)
VI  - 72
IP  - 3
DP  - 1983 Mar
TI  - High-performance liquid chromatography of preparations of ribonucleic acid 
      inactivator(s) from cupric ion and hydroquinone before and after treatment with 
      histidine.
PG  - 300-3
AB  - Preparations of viral RNA inactivator(s) produced during the cupric ion-catalyzed 
      oxidation of hydroquinone were analyzed by high-performance liquid chromatography 
      (HPLC) using UV and electrochemical (EC) detectors. In addition to hydroquinone 
      and the main oxidation product (p-benzoquinone), which is known not to be the 
      inactivator(s), the analysis showed three unidentified components (I-III). 
      Partial UV absorption spectra of I-III were determined by HPLC with the UV 
      detector set at various wavelengths. Components II and III, but not I, were 
      highly unstable in the presence of L-histidine, which is an excellent chelator of 
      cupric ion and can promptly stop ongoing viral RNA inactivation by the 
      inactivator(s). The product p-benzoquinone was also highly unstable in the 
      presence of L-histidine; the reaction between these two compounds (with or 
      without copper) resulted in a cascade of products. The possibility that the 
      inactivator(s) is II or III, or both, is discussed.
FAU - Dubes, G R
AU  - Dubes GR
FAU - Masoud, A N
AU  - Masoud AN
FAU - Al-Moslih, M I
AU  - Al-Moslih MI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pharm Sci
JT  - Journal of pharmaceutical sciences
JID - 2985195R
RN  - 0 (Hydroquinones)
RN  - 0 (RNA, Viral)
RN  - 4QD397987E (Histidine)
RN  - 789U1901C5 (Copper)
RN  - XV74C1N1AE (hydroquinone)
SB  - IM
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Chromatography, High Pressure Liquid/methods
MH  - *Copper
MH  - *Histidine
MH  - *Hydroquinones
MH  - RNA, Viral/*antagonists & inhibitors
MH  - Spectrophotometry, Ultraviolet
EDAT- 1983/03/01 00:00
MHDA- 1983/03/01 00:01
CRDT- 1983/03/01 00:00
PHST- 1983/03/01 00:00 [pubmed]
PHST- 1983/03/01 00:01 [medline]
PHST- 1983/03/01 00:00 [entrez]
AID - S0022-3549(15)44543-5 [pii]
AID - 10.1002/jps.2600720323 [doi]
PST - ppublish
SO  - J Pharm Sci. 1983 Mar;72(3):300-3. doi: 10.1002/jps.2600720323.