PMID- 6883834
OWN - NLM
STAT- MEDLINE
DCOM- 19831028
LR  - 20061115
IS  - 0147-958X (Print)
IS  - 0147-958X (Linking)
VI  - 6
IP  - 2
DP  - 1983
TI  - Absence of circulating immune complexes containing DNA in hemodialysed sera.
PG  - 73-7
AB  - Although the presence of free DNA and antibodies to DNA in the sera of patients 
      on maintenance hemodialysis has been reported, we found only a low incidence of 
      antibodies to single-stranded DNA (ss-DNA) and native DNA (n-DNA) using an 
      improved detection technique. In our search for evidence of the possible 
      formation of DNA-anti-DNA circulating immune complexes (CIC), antibodies to n-DNA 
      were detected in the sera of only 2 of 53 dialysed and 1 of 25 non-dialysed renal 
      patients. CICs were detected in the sera of 17 of 53 dialysed (28% by Raji-RIA, 
      6% by Clq-BA) and 6 of 25 non-dialysed (20% by Raji-RIA and 24% by Clq-BA) renal 
      patients: the difference is not significant (P greater than 0.25). DNase 
      digestion of the dialysed sera that were positive for CIC by Raji-RIA produced no 
      significant change in the results on re-assay (P greater than 0.50) in contrast 
      with a significant decrease in CIC after DNase digestion of selected SLE sera and 
      of solutions containing DNA-anti-DNA complexes formed in vitro (P less than 
      0.05). The formation of circulating DNA-anti-DNA complexes appears not to be the 
      cause of the low incidence of antibodies to n-DNA in the sera of dialysed 
      patients.
FAU - Dasgupta, M K
AU  - Dasgupta MK
FAU - Davis, P
AU  - Davis P
FAU - Johny, K V
AU  - Johny KV
FAU - Higgins, M R
AU  - Higgins MR
FAU - Dossetor, J B
AU  - Dossetor JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Clin Invest Med
JT  - Clinical and investigative medicine. Medecine clinique et experimentale
JID - 7804071
RN  - 0 (Antibodies)
RN  - 0 (Antigen-Antibody Complex)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibodies/analysis
MH  - Antigen-Antibody Complex/*analysis
MH  - DNA/*immunology
MH  - Humans
MH  - Kidney Failure, Chronic/immunology/therapy
MH  - *Renal Dialysis
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
PST - ppublish
SO  - Clin Invest Med. 1983;6(2):73-7.