PMID- 6894870
OWN - NLM
STAT- MEDLINE
DCOM- 19811029
LR  - 20181113
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 29
IP  - 1
DP  - 1980 Jan
TI  - Dynamic light-scattering evidence for the flexibility of native muscle thin 
      filaments.
PG  - 37-47
AB  - We have obtained clear evidence for the flexibility of native scallop adductor 
      thin filaments by studying the temperature and ionic strength dependence of the 
      average decay constants obtained from intensity fluctuation spectroscopic (IFS) 
      measurements. The low-angle (10-25 degrees ), average decay constants obtained 
      from time autocorrelation functions of scattered light were independent of 
      concentration (0.08-1.3 mg/ml), scaled with the ratio of temperature to solvent 
      viscosity, T/eta, over a range of 4-45 degrees C, and yielded a value for the 
      translational diffusion coefficient of D(T) (5 degrees C) = (1.24 +/- 0.06) x 
      10(-8) cm(2)/s. From this value and the Broersma relation for rigid rods, we find 
      an average filament length of 1.06 +/- 0.06 mum. Quantitative sodium dodecyl 
      sulfate polyacrylamide gel electrophoresis showed that at high temperatures (> 35 
      degrees C) or in 0.6 M NaCl, tropomyosin completely dissociates from native thin 
      filaments. Decay constants from high-angle (60-150 degrees C) IFS temperature 
      dependence measurements do not scale with T/eta and hence do not show the 
      temperature dependence expected for rigid rods. The differences are not due to 
      any change in length distribution of filaments with temperature or to the free 
      tropomyosin in solution, but are attributed to nonrigid motions of the filaments. 
      Similar experiments on samples in high- and low-salt solvents gave results 
      consistent with this interpretation.
FAU - Newman, J
AU  - Newman J
FAU - Carlson, F D
AU  - Carlson FD
LA  - eng
GR  - AM-12803/AM/NIADDK NIH HHS/United States
GR  - AM-16315/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Actins)
RN  - 0 (Tropomyosin)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Light
MH  - Mollusca/*anatomy & histology
MH  - Muscles/*anatomy & histology/metabolism
MH  - Osmolar Concentration
MH  - Scattering, Radiation
MH  - Sodium Chloride/metabolism
MH  - Temperature
MH  - Tropomyosin/metabolism
PMC - PMC1328660
EDAT- 1980/01/01 00:00
MHDA- 1980/01/01 00:01
PMCR- 1981/01/01
CRDT- 1980/01/01 00:00
PHST- 1980/01/01 00:00 [pubmed]
PHST- 1980/01/01 00:01 [medline]
PHST- 1980/01/01 00:00 [entrez]
PHST- 1981/01/01 00:00 [pmc-release]
AID - S0006-3495(80)85116-2 [pii]
AID - 10.1016/S0006-3495(80)85116-2 [doi]
PST - ppublish
SO  - Biophys J. 1980 Jan;29(1):37-47. doi: 10.1016/S0006-3495(80)85116-2.