PMID- 6953160
OWN - NLM
STAT- MEDLINE
DCOM- 19820807
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 93
IP  - 2
DP  - 1982 May
TI  - Cortisol:cortisone interconversion by human decidua in relation to parturition: 
      effect of tissue manipulation on 11 beta-hydroxysteroid dehydrogenase activity.
PG  - 141-9
AB  - Cortisol:cortisone interconversion was studied in human decidua obtained from 
      three groups of patients at term (37-42 weeks): before the onset of labour (at 
      elective Caesarean section), after labour of spontaneous onset, and after labour 
      of induced onset. When intact tissue was incubated with [3H]cortisol or 
      [3H]cortisone in phosphate buffer without added substrate or cofactors, cortisone 
      to cortisol was the dominant conversion. However, when damaged cells or tissue 
      homogenates were used in the same conditions, the dominant direction of the 
      reaction was reversed, with a large increase in oxidative (cortisol to cortisone) 
      activity. Cortisol:cortisone interconversion was similar in the three groups of 
      samples using either intact tissue or homogenates, as was the total 11 
      beta-hydroxysteroid dehydrogenase (11 beta-HSD) activity measured in tissue 
      homogenates in the presence of added substrate (cortisol or cortisone) and 
      cofactors (NADP+ or NADPH). Endogenous cortisol concentrations in decidua were 
      higher than those of cortisone, and the ratio of cortisol to cortisone was 
      similar in the three groups. These findings suggest that there are no changes in 
      human decidual 11 beta-HSD activity in relation to parturition. Specific activity 
      of 11 beta-HSD decreased at high protein concentrations, suggesting the presence 
      of some enzyme inhibitor(s) in homogenized decidual tissue.
FAU - Lopez Bernal, A
AU  - Lopez Bernal A
FAU - Anderson, A B
AU  - Anderson AB
FAU - Turnbull, A C
AU  - Turnbull AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - V27W9254FZ (Cortisone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Cortisone/*metabolism
MH  - Decidua/enzymology/*metabolism
MH  - Female
MH  - Humans
MH  - Hydrocortisone/*metabolism
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - In Vitro Techniques
MH  - Labor, Induced
MH  - *Labor, Obstetric
MH  - Pregnancy
MH  - Radioimmunoassay
EDAT- 1982/05/01 00:00
MHDA- 1982/05/01 00:01
CRDT- 1982/05/01 00:00
PHST- 1982/05/01 00:00 [pubmed]
PHST- 1982/05/01 00:01 [medline]
PHST- 1982/05/01 00:00 [entrez]
AID - 10.1677/joe.0.0930141 [doi]
PST - ppublish
SO  - J Endocrinol. 1982 May;93(2):141-9. doi: 10.1677/joe.0.0930141.