PMID- 6967945
OWN - NLM
STAT- MEDLINE
DCOM- 19801124
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 152
IP  - 3
DP  - 1980 Sep 1
TI  - Augmentation of in vitro humoral immune responses in the mouse by an antibody to 
      IgD.
PG  - 493-506
AB  - Heterologous anti-delta-chain antibodies have an adjuvant effect on specific in 
      vivo humoral immune responses to simultaneously, or subsequently, injected 
      antigens in the rat and rhesus monkey. We have used a hybridoma-secreted antibody 
      that binds murine delta-chain of the allotype (4.22aM delta a) to study this 
      phenomenon in the mouse and to investigate the mechanism of this effect. 
      Injection of 4.22aM delta a into BALB/c mice removes almost all surface IgD 
      (sIgD) from splenic B lymphocites. sIgD does not reappear until the serum level 
      of 4.22aM delta a decreased 5-7 d after injection. 4.22aM delta a fails to induce 
      detectable proliferation or to raise total serum Ig levels substantially above 
      control values. However, 4.22aM dalta a injected 24 h before antigen elicits an 
      approximately twofold enhancement of serum IgM and a 3- to 10-fold enhancement of 
      serum IgG anti-trintriphenyl (TNP) antibodies in response to immunization with 
      optimal doses of TNP-Ficoll or TNP-sheep red blood cells (TNP-SRBC). 4.22aM delta 
      a injected 1 wk before or 3 d after TNP-SRBC, however, has no effect on IgG 
      anti-TNP levels. The adjuvant effect of anti-delta-chain antibody was markedly 
      decreased when suboptimal antigen doses were used. Furthermore, even in the case 
      of TNP-Ficoll, a relatively T-independent antigen, the ability of 4.22aM dalta a 
      to enhance the anti-TNP antibody response was T cell dependent. Our data suggest 
      that the binding of anti-delta-chain antibody to cell sIgD may partially activate 
      B lymphocytes and make them more capable of differentiating into 
      antibody-secreting cells when stimulated by antigen-specific T cell help.
FAU - Finkelham, F D
AU  - Finkelham FD
FAU - Woods, V L
AU  - Woods VL
FAU - Wilburn, S B
AU  - Wilburn SB
FAU - Mond, J J
AU  - Mond JJ
FAU - Stein, K E
AU  - Stein KE
FAU - Berning, A
AU  - Berning A
FAU - Scher, I
AU  - Scher I
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Animals
MH  - *Antibodies, Anti-Idiotypic
MH  - *Antibody Formation
MH  - B-Lymphocytes/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - *Immunoglobulin D
MH  - Immunoglobulin G/biosynthesis
MH  - Immunoglobulin M/biosynthesis
MH  - *Immunologic Memory
MH  - Lymphocyte Cooperation
MH  - Mice
MH  - Receptors, Antigen, B-Cell/*immunology
MH  - T-Lymphocytes/immunology
PMC - PMC2185918
EDAT- 1980/09/01 00:00
MHDA- 1980/09/01 00:01
PMCR- 1981/03/01
CRDT- 1980/09/01 00:00
PHST- 1980/09/01 00:00 [pubmed]
PHST- 1980/09/01 00:01 [medline]
PHST- 1980/09/01 00:00 [entrez]
PHST- 1981/03/01 00:00 [pmc-release]
AID - 81008373 [pii]
AID - 10.1084/jem.152.3.493 [doi]
PST - ppublish
SO  - J Exp Med. 1980 Sep 1;152(3):493-506. doi: 10.1084/jem.152.3.493.