PMID- 6969570
OWN - NLM
STAT- MEDLINE
DCOM- 19810219
LR  - 20131121
IS  - 0091-7370 (Print)
IS  - 0091-7370 (Linking)
VI  - 10
IP  - 6
DP  - 1980 Nov-Dec
TI  - B and T lymphocytes: quantitation, function, and clinical applicability.
PG  - 455-62
AB  - Methodologies for T and B lymphocyte quantitation, lymphocyte blast 
      transformation (LBT) and carbohydrate (CHO) metabolism are important for 
      assessing host lymphocyte response in the clinical laboratory. Modifications of 
      methods for each of these techniques are presented. Results from studies of 
      normal ambulatory adults, patients with diabetes mellitus, sickle cell disease 
      and hyperlipidemia are reported. LBT of normal lymphocytes before and after 
      ethanol exposure are examined. LBT during pregnancy is evaluated. T cell 
      populations are abnormally high in black diabetics and decreased in patients with 
      sickle cell anemia. B cell subpopulations are increased in patients with sickle 
      cell anemia. LBT responses are decreased in maturity onset diabetes, during 
      pregnancy and in patients with sickle cell disease. Ethanol in amounts attainable 
      during human consumption results in significantly decreased LBT response. CHO 
      metabolism (especially hexose monophosphate shunt [HMPS] and HMPS by pentose 
      sugar recycling) is abnormal in diabetic lymphocytes. The low HMPS activity is 
      partially reversible by treatment with prostaglandin synthetase inhibitors. 
      Information related to lymphocytes in normal states remains to be collected by 
      further clinical application of these techniques of quantitation and in vitro 
      function.
FAU - Glassman, A B
AU  - Glassman AB
FAU - Bennett, C E
AU  - Bennett CE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Hexosephosphates)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Anemia, Sickle Cell/blood
MH  - B-Lymphocytes/*physiology
MH  - Citric Acid Cycle
MH  - Diabetes Mellitus/blood
MH  - Female
MH  - Glucose/*metabolism
MH  - Hexosephosphates/metabolism
MH  - Humans
MH  - *Leukocyte Count
MH  - *Lymphocyte Activation
MH  - Male
MH  - T-Lymphocytes/*physiology
EDAT- 1980/11/01 00:00
MHDA- 1980/11/01 00:01
CRDT- 1980/11/01 00:00
PHST- 1980/11/01 00:00 [pubmed]
PHST- 1980/11/01 00:01 [medline]
PHST- 1980/11/01 00:00 [entrez]
PST - ppublish
SO  - Ann Clin Lab Sci. 1980 Nov-Dec;10(6):455-62.