PMID- 6972806
OWN - NLM
STAT- MEDLINE
DCOM- 19810915
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 217
IP  - 1
DP  - 1981
TI  - Lymphocyte stimulation by concanavalin A studied by the fluorescent probe 
      acridine orange.
PG  - 117-26
AB  - Viable mouse thymocytes or spleen leucocytes stained with acridine orange (AO) 
      were divided into one part used for stimulation, and the other part for control. 
      Analysis of cellular green-fluorescence emission enabled physicochemical changes 
      in lymphocytes to be detected after 30 min stimulation with the mitogens 
      concanavalin A (Con A) and pokeweed mitogen (PWM). No change in fluorescence was 
      observed with the nonmitogenic reagent wheat germ lectin (WGL) or with allogeneic 
      cell stimulation (MLR). When green fluorescence intensity of individual cells was 
      monitored by microfluorimetry, 30 min stimulation with Con A induced an increase, 
      whereas PWM induced a decrease. When analysed by fluorescence spectrophotometry, 
      Con A induced a 2 nm blue shift in emission maximum and a decrease in 
      polarization values.
FAU - Halliday, G M
AU  - Halliday GM
FAU - Nairn, R C
AU  - Nairn RC
FAU - Rolland, J M
AU  - Rolland JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Azides)
RN  - 0 (Pokeweed Mitogens)
RN  - 11028-71-0 (Concanavalin A)
RN  - 968JJ8C9DV (Sodium Azide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Azides/pharmacology
MH  - Concanavalin A/*pharmacology
MH  - *Lymphocyte Activation/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Pokeweed Mitogens/pharmacology
MH  - Sodium Azide
MH  - Spectrometry, Fluorescence
MH  - T-Lymphocytes/*immunology
EDAT- 1981/01/01 00:00
MHDA- 1981/01/01 00:01
CRDT- 1981/01/01 00:00
PHST- 1981/01/01 00:00 [pubmed]
PHST- 1981/01/01 00:01 [medline]
PHST- 1981/01/01 00:00 [entrez]
AID - 10.1007/BF00233831 [doi]
PST - ppublish
SO  - Cell Tissue Res. 1981;217(1):117-26. doi: 10.1007/BF00233831.