PMID- 6980685 OWN - NLM STAT- MEDLINE DCOM- 19821021 LR - 20190503 IS - 0007-134X (Print) IS - 0007-134X (Linking) VI - 58 IP - 4 DP - 1982 Aug TI - Isoenzyme characterisation of Trichomonas vaginalis. PG - 250-6 AB - Clones of 32 strains of Trichomonas vaginalis isolated from patients attending a venereal diseases clinic were compared among themselves and with authentic Pentatrichomonas hominis on the basis of their isoenzyme patterns for eight enzymes by thin-layer starch-gel electrophoresis. The enzymes examined were: glucose phosphate isomerase (GPI); phosphoglucomutase (PGM); malic enzyme (NADP+) (ME); hexokinase (HK); malate dehydrogenase (NAD+) (MDH); glucose-6-phosphate dehydrogenase (G6PD); aldolase (ALD); and lactate dehydrogenase (LDH). From the isoenzyme patterns of four enzymes (LDH, MDH, HK, and GPI) the strains of T vaginalis could be divided clearly into five groups. PGM showed differences in only one strain, while two other enzyme patterns (ME and ALD) were the same for all the strains of T vaginalis tested. All isolates were clearly distinguishable from P hominis. Although G6PD patterns were not sharp some differences were evident among T vaginalis strains. FAU - Soliman, M A AU - Soliman MA FAU - Ackers, J P AU - Ackers JP FAU - Catterall, R D AU - Catterall RD LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Br J Vener Dis JT - The British journal of venereal diseases JID - 0421042 RN - 0 (Isoenzymes) SB - IM MH - Animals MH - Electrophoresis, Starch Gel MH - Female MH - Humans MH - Isoenzymes/*analysis MH - Mice MH - Trichomonas/classification/enzymology MH - Trichomonas vaginalis/classification/*enzymology PMC - PMC1046060 EDAT- 1982/08/01 00:00 MHDA- 1982/08/01 00:01 PMCR- 1982/08/01 CRDT- 1982/08/01 00:00 PHST- 1982/08/01 00:00 [pubmed] PHST- 1982/08/01 00:01 [medline] PHST- 1982/08/01 00:00 [entrez] PHST- 1982/08/01 00:00 [pmc-release] AID - 10.1136/sti.58.4.250 [doi] PST - ppublish SO - Br J Vener Dis. 1982 Aug;58(4):250-6. doi: 10.1136/sti.58.4.250.