PMID- 6995331
OWN - NLM
STAT- MEDLINE
DCOM- 19801021
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 29
IP  - 1
DP  - 1980 Jul
TI  - Detection of Escherichia coli enterotoxins in stools.
PG  - 108-13
AB  - We determined whether enterotoxigenic Escherichia coli diarrhea could be 
      diagnosed by direct examination of stools for heat-labile (LT) and heat-stable 
      (ST) enterotoxins. The Y-1 adrenal cell and an enzyme-linked immunosorbent assay 
      (ELISA) detected LT in 85 and 93%, respectively, of stool specimens obtained from 
      adults with acute diarrhea from whom an LT- and ST-producing organism had been 
      isolated. Furthermore, the ELISA assay detected LT in 8 of 35 stool specimens 
      from which no LT-producing E. coli had been isolated. The infant mouse assay was 
      utilized to detect ST in these stool specimens and was found to be an insensitive 
      method, showing positive results in only 36% of the specimens from which an 
      ST-producing organism was isolated. Further studies are warranted to determine 
      the diagnostic value of direct detection of LT in stools, especially by the ELISA 
      method.
FAU - Merson, M H
AU  - Merson MH
FAU - Yolken, R H
AU  - Yolken RH
FAU - Sack, R B
AU  - Sack RB
FAU - Froehlich, J L
AU  - Froehlich JL
FAU - Greenberg, H B
AU  - Greenberg HB
FAU - Huq, I
AU  - Huq I
FAU - Black, R W
AU  - Black RW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Bacterial Toxins/*analysis
MH  - Biological Assay
MH  - Cell Line
MH  - Child
MH  - Child, Preschool
MH  - Diarrhea/etiology
MH  - Enterotoxins/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Escherichia coli
MH  - Escherichia coli Infections/diagnosis
MH  - Feces/*analysis
MH  - Female
MH  - Hot Temperature
MH  - Humans
MH  - Infant
MH  - Male
MH  - Mice
MH  - Middle Aged
PMC - PMC551081
EDAT- 1980/07/01 00:00
MHDA- 1980/07/01 00:01
PMCR- 1980/07/01
CRDT- 1980/07/01 00:00
PHST- 1980/07/01 00:00 [pubmed]
PHST- 1980/07/01 00:01 [medline]
PHST- 1980/07/01 00:00 [entrez]
PHST- 1980/07/01 00:00 [pmc-release]
AID - 10.1128/iai.29.1.108-113.1980 [doi]
PST - ppublish
SO  - Infect Immun. 1980 Jul;29(1):108-13. doi: 10.1128/iai.29.1.108-113.1980.