PMID- 7007526
OWN - NLM
STAT- MEDLINE
DCOM- 19810424
LR  - 20190511
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 142
IP  - 6
DP  - 1980 Dec
TI  - Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
PG  - 892-8
AB  - A method fo detecting large numbers of isolates of enterotoxigenic Escherichia 
      coli is described in which the genes encoding th enterotoxins are detected, 
      rather than the toxins themselves. Radiolabeled fragments of DNA encoding the 
      heat-labile (LT) or heat-stable (ST) toxins were used as hybridization probes for 
      homologous DNA sequences in E. coli colonies grown and lysed in situ on 
      nitrocellulose filters. The LT probe detected all of 31 E. coli strains producing 
      ST and LT or only LT, while the ST probe detected 12 of 17 strains producing only 
      ST and three of 26 strains producing ST and LT. These results suggest that the 
      LTs produced by different isolates of E. coli are homologous and that human 
      isolates of E. coli produce at least two heterologous STs detectable in the 
      infant mouse assay. The hybridization method also detected the presence of 
      enterotoxigenic E. coli in bacterial growth in directly spotted stools from 
      patients with acute diarrhea.
FAU - Moseley, S L
AU  - Moseley SL
FAU - Huq, I
AU  - Huq I
FAU - Alim, A R
AU  - Alim AR
FAU - So, M
AU  - So M
FAU - Samadpour-Motalebi, M
AU  - Samadpour-Motalebi M
FAU - Falkow, S
AU  - Falkow S
LA  - eng
GR  - AI 07149-02/AI/NIAID NIH HHS/United States
GR  - AI 10885-08/AI/NIAID NIH HHS/United States
GR  - DADA 17-72-C2149/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Acute Disease
MH  - DNA, Bacterial/*genetics
MH  - Diarrhea/microbiology
MH  - Enterotoxins/*genetics
MH  - Escherichia coli/*genetics
MH  - Escherichia coli Infections/microbiology
MH  - Genetic Code
MH  - Humans
MH  - *Hybridization, Genetic
EDAT- 1980/12/01 00:00
MHDA- 1980/12/01 00:01
CRDT- 1980/12/01 00:00
PHST- 1980/12/01 00:00 [pubmed]
PHST- 1980/12/01 00:01 [medline]
PHST- 1980/12/01 00:00 [entrez]
AID - 10.1093/infdis/142.6.892 [doi]
PST - ppublish
SO  - J Infect Dis. 1980 Dec;142(6):892-8. doi: 10.1093/infdis/142.6.892.