PMID- 702335
OWN - NLM
STAT- MEDLINE
DCOM- 19781227
LR  - 20141120
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 207
IP  - 1
DP  - 1978 Oct
TI  - The effect of delta9-tetrahydrocannabinol on the conversion of [3H]trypotphan to 
      5-[3H] hydroxytryptamine in the mouse brain.
PG  - 140-50
AB  - The effects of a 30-min pretreatment with varying doses of 
      delta9-tetrahydrocannibinol (delta9-THC) on the synthesis of 
      5-[3H]hydroxytryptamine (5-[3H]HT) from an intravenous 10-min pulse of 
      L-[3H]tryptophan ([3H]try) were measured in the mouse brain. We also determined 
      the effects of delta9-THC on several parameters believed to influence the 
      synthesis of brain 5-HT, including total and free plasma tryptophan and the 
      high-affinity synaptosomal uptake of tryptophan. Delta9-THC was found to increase 
      the amount of [3H]try accumulated by the brain as well as the amount of 5-[3H]HT 
      synthesized. This effect was greater at intermediate doses than at the highest 
      dose tested. However, delta9-THC was determined to have no effect on the actual 
      rate of conversion of [3H]try into 5-[3H]HT at any dose tested. Delta9-THC had no 
      effect on either plasma tyrosine ot free tryptophan levels. However, delta9-THC 
      decreased total plasma tryptophan at low and intermediate doses, but had no 
      effect at the highest dose tested. Synaptosomal uptake of [3H]try was unaffected 
      by pretreatment with delta9-THC at any dose tested. These data suggest that 
      delta9-THC increases the synthesis of 5-HT, not by altering the actual rate of 
      conversion of tryptophan to 5-HT, but by altering, via an unknown mechanism, the 
      quantity of tryptophan available for conversion to 5-HT.
FAU - Johnson, K M
AU  - Johnson KM
FAU - Dewey, W L
AU  - Dewey WL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 333DO1RDJY (Serotonin)
RN  - 42HK56048U (Tyrosine)
RN  - 7J8897W37S (Dronabinol)
RN  - 8DUH1N11BX (Tryptophan)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/*metabolism
MH  - Dronabinol/*pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Serotonin/*biosynthesis
MH  - Synaptosomes/drug effects/metabolism
MH  - Tryptophan/blood/*metabolism
MH  - Tyrosine/blood
EDAT- 1978/10/01 00:00
MHDA- 1978/10/01 00:01
CRDT- 1978/10/01 00:00
PHST- 1978/10/01 00:00 [pubmed]
PHST- 1978/10/01 00:01 [medline]
PHST- 1978/10/01 00:00 [entrez]
PST - ppublish
SO  - J Pharmacol Exp Ther. 1978 Oct;207(1):140-50.