PMID- 7024956
OWN - NLM
STAT- MEDLINE
DCOM- 19811122
LR  - 20190913
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 1
IP  - 4
DP  - 1980
TI  - Immunohistological approaches to human prostatic epithelial cells.
PG  - 383-98
AB  - Specific anti-acid phosphatase antisera were prepared by the use of purified 
      prostatic acid phosphatase (PAP) from prostatic tissue and lysosomal acid 
      phosphatase (L-AcP) from diploid fibroblast (WI-38) as antigen. Since the 
      antigenic difference between the PAP and L-AcP was established, distribution of 
      these enzymes among various tissues and tissue culture cells was investigated by 
      the indirect immunofluorescence method and the unlabeled antibody method [14]. 
      The L-AcP has been detected as granules localized in a perinuclear portion of all 
      of the epithelial cells, fibroblasts, and peripheral blood cells except 
      erythrocytes. Presence of the PAP was limited to the prostatic epithelial cells. 
      It is also noted that the biosynthesis of the PAP is strikingly diminished in the 
      long-term culture of prostatic epithelial cells. However, because of the 
      antigenic difference between the PAP and L-AcP, and because of the high 
      concentration of PAP in the cytoplasm of prostatic epithelial cells, it is 
      feasible to apply the anti-PAP immunofluorescence method to the identification of 
      metastatic prostatic carcinoma cells in the biopsy materials.
FAU - Choe, B K
AU  - Choe BK
FAU - Pontes, J E
AU  - Pontes JE
FAU - Lillehoj, H S
AU  - Lillehoj HS
FAU - Rose, N R
AU  - Rose NR
LA  - eng
GR  - CA16426/CA/NCI NIH HHS/United States
GR  - CA18748/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - EC 3.1.3.2 (Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/*immunology
MH  - Cell Line
MH  - Epithelial Cells
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Molecular Weight
MH  - Prostate/*cytology/immunology
EDAT- 1980/01/01 00:00
MHDA- 1980/01/01 00:01
CRDT- 1980/01/01 00:00
PHST- 1980/01/01 00:00 [pubmed]
PHST- 1980/01/01 00:01 [medline]
PHST- 1980/01/01 00:00 [entrez]
AID - 10.1002/pros.2990010402 [doi]
PST - ppublish
SO  - Prostate. 1980;1(4):383-98. doi: 10.1002/pros.2990010402.