PMID- 7045323
OWN - NLM
STAT- MEDLINE
DCOM- 19820814
LR  - 20061115
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 53
IP  - 5
DP  - 1982 May
TI  - Evaluation of the immunogenicity of freeze-dried skin allografts in humans: 
      cell-mediated response.
PG  - 325-7
AB  - PATIENTS RECEIVING freeze-dried skin (FDS) allografts were evaluated for 
      cell-mediated response by a lymphocytotoxicity test. Eleven patients received 
      single or multiple FDS allografts from a donor typed for human leukocyte antigen 
      (HLA). Heparinized blood samples were drawn before the procedure and at 2, 4, and 
      8 weeks after grafting. Mononuclear cells were isolated by Ficoll-Hypaque 
      gradients. Cell-mediated lymphocytotoxicity tests were conducted, with 
      mononuclear cells from the FDS allograft recipients used as effector cells. 
      Phytohemagglutinin-stimulated blast target cells of the same HLA type as that of 
      the skin donor were labeled with chromium 51. Lympholysis was evaluated by 
      measuring the amount of 51Cr release after 18 hours' incubation with 
      target/effector cell ratios of 1:100, 1:50, and 1:25. Negative control wells 
      contained target cells alone. Positive control wells contained target cells and 
      effector cells sensitized in vitro against the target cells. Viability of 
      effector cells was tested by trypan-blud dye exclusion and response to 
      phytohemagglutinin. None of the experimental blood samples showed 51Cr release 
      significantly greater than shown by the negative controls. Our findings of no 
      cell-mediated response and the findings of a previous study showing no production 
      of anti-HLA antibody in response to FDS allografts indicate that allogeneic FDS 
      is an immunologically safe material for use in periodontal surgical procedures.
FAU - Gher, M E Jr
AU  - Gher ME Jr
FAU - Vernino, A R
AU  - Vernino AR
FAU - Maluish, A E
AU  - Maluish AE
FAU - Strong, D M
AU  - Strong DM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
SB  - IM
MH  - Cytotoxicity, Immunologic
MH  - Freeze Drying
MH  - Graft Rejection
MH  - Humans
MH  - Hypersensitivity, Delayed/immunology
MH  - *Immunity, Cellular
MH  - Lymphocytes/immunology
MH  - Periodontal Diseases/surgery
MH  - *Skin Transplantation
MH  - Time Factors
MH  - *Transplantation Immunology
MH  - Transplantation, Homologous
EDAT- 1982/05/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1982/05/01 00:00
PHST- 1982/05/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1982/05/01 00:00 [entrez]
AID - 10.1902/jop.1982.53.5.325 [doi]
PST - ppublish
SO  - J Periodontol. 1982 May;53(5):325-7. doi: 10.1902/jop.1982.53.5.325.