PMID- 7058898
OWN - NLM
STAT- MEDLINE
DCOM- 19820420
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 242
IP  - 1
DP  - 1982 Jan
TI  - Migrating action-potential complex activity in absence of fluid production is 
      produced by B subunit of cholera enterotoxin.
PG  - G47-51
AB  - The myoelectric response and fluid output from in vivo rabbit ileal loops 
      injected with B subunits of purified cholera enterotoxin are compared with the 
      response to the purified cholera holotoxin. Migrating action-potential complex 
      (MAPC) frequency is similar after injection of purified cholera toxin or B 
      subunits. In contrast, fluid output after B-subunit injection is significantly (P 
      less than 0.05) less than fluid output after purified cholera toxin injection. 
      Specific antiserum to the holotoxin incubated with holotoxin at equivalence 
      significantly decreased both MAPC activity (P less than 0.05) and fluid output (P 
      less than 0.001) from the purified toxin. Purified cholera toxin and B subunits, 
      modified by reaction with 2-nitrophenylsulfonyl chloride to produce monomeric B 
      fractions with decreased GM1 ganglioside-binding properties, produced 
      significantly (P less than 0.05) less fluid output and MAPC activity. Binding of 
      the toxin or B subunits in the aggregated form is essential for the expression of 
      MAPC activity or fluid output. These results suggest that the B subunit of 
      cholera toxin produces MAPC activity in the rabbit ileum in the absence of fluid 
      production. Furthermore, previous assumptions that MAPC activity is linked to 
      fluid secretion should be reconsidered. It appears that the gut responses to 
      cholera toxin, fluid production, or MAPC activity are produced by separate 
      mechanisms.
FAU - Sinar, D R
AU  - Sinar DR
FAU - Charles, L G
AU  - Charles LG
FAU - Burns, T W
AU  - Burns TW
LA  - eng
GR  - R08317/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Immune Sera)
RN  - 0 (Macromolecular Substances)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Cholera Toxin/*pharmacology
MH  - Ileum/drug effects/*physiology
MH  - Immune Sera
MH  - Macromolecular Substances
MH  - Rabbits
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1152/ajpgi.1982.242.1.G47 [doi]
PST - ppublish
SO  - Am J Physiol. 1982 Jan;242(1):G47-51. doi: 10.1152/ajpgi.1982.242.1.G47.