PMID- 7070578
OWN - NLM
STAT- MEDLINE
DCOM- 19820614
LR  - 20190821
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 7
IP  - 1
DP  - 1982 Jan
TI  - Modifications in energy metabolism during the development of chick glial cells 
      and neurons in culture.
PG  - 27-36
AB  - Developmental changes in lactate dehydrogenase (LDH), enolase, hexokinase (HK), 
      malate dehydrogenase (MDH), and glutamate dehydrogenase (GDH) activities were 
      measured in cultures of pure neurons and glial cells prepared from brains of 
      chick embryos (8 day-old for neurons, 14 day-old for glial cells) as a function 
      of cellular development with time in culture. The modifications observed in 
      culture were compared to those measured in brain extracts during the development 
      of the nervous tissue in the chick embryo and during the post-hatching period. A 
      significant increase of MDH, GDH, LDH, and enolase activities are observed in 
      neurons between 3 and 6 days of culture, whereas simultaneously a decrease of HK 
      values occurs. In the embryonic brain between 11 and 14 days of incubation, which 
      would correspond for the neuronal cultures to day 3 through 6, modifications of 
      MDH, GDH, HK, and enolase levels are similar to those observed in neurons in 
      culture. Only the increase of LDH activity is less pronounced in vivo than in 
      cultivated cells. The evolution of the tested enzymatic activities in the brain 
      of the chick during the period between 7 days before and 10 days after hatching 
      is quite similar to that observed in cultivated glial cells (prepared from 14 
      day-old embryos) between 6 and 18 days of culture. All tested activities 
      increased in comparable proportions. The modifications of the enzymatic profile 
      indicate that some maturation phenomena affecting energy metabolism of neuronal 
      and glial elements in culture, are quite similar to those occuring in the total 
      nervous tissue. A relationship between the development of the energy metabolism 
      of the brain and differentiation processes affecting neuroblasts and the 
      glial-forming cells is discussed.
FAU - Tholey, G
AU  - Tholey G
FAU - Ledig, M
AU  - Ledig M
FAU - Mandel, P
AU  - Mandel P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - Brain/*enzymology
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - *Energy Metabolism
MH  - Glutamate Dehydrogenase/metabolism
MH  - Hexokinase/metabolism
MH  - Kinetics
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Malate Dehydrogenase/metabolism
MH  - Neuroglia/*enzymology
MH  - Neurons/*enzymology
MH  - Phosphopyruvate Hydratase/metabolism
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1007/BF00965066 [doi]
PST - ppublish
SO  - Neurochem Res. 1982 Jan;7(1):27-36. doi: 10.1007/BF00965066.