PMID- 7074225
OWN - NLM
STAT- MEDLINE
DCOM- 19820722
LR  - 20151119
IS  - 0303-6847 (Print)
IS  - 0303-6847 (Linking)
VI  - 19
IP  - 3
DP  - 1982
TI  - Characteristics of hormone-stimulated adenylate cyclase in vascular smooth 
      muscle: altered activity in spontaneously hypertensive rat.
PG  - 109-16
AB  - The stimulation of adenylate cyclase activity by guanine nucleotides, 
      isoproterenol and fluoride has been investigated in KCl washed rat aortic 
      membranes. We observed that the presence of guanine nucleotides in the assay was 
      obligatory for the isoproterenol stimulation of adenylate cyclase activity. 
      Guanine nucleotides could stimulate adenylate cyclase in the absence of 
      catecholamines; however, the extent of stimulation was significantly lower (p 
      less than 0.05) than that observed with isoproterenol. The order of effectiveness 
      of guanine nucleotides was guanosine 5'-0-(3-thiotriphosphate) (GTP-gamma-S), 
      5'-guanylylimido diphosphate (Gpp(NH)p), and guanosine 5'-triphosphate (GTP). GTP 
      stimulated enzyme activity without a noticeable lag period, while stimulation by 
      Gpp(NH)p showed a lag period of 3-4 min. Addition of isoproterenol neither 
      abolished the lag period, nor altered the apparent K1 value (concentration 
      required for half maximal stimulation) for Gpp(NH)p stimulation. In a comparative 
      study of adenylate cyclase activity between spontaneously hypertensive rats (SHR) 
      and Kyoto Wistar normotensive rats (WKY), using 0.6 M KCl washed membranes from 
      aorta and caudal artery, no differences were observed in the basal adenylate 
      cyclase activity. However, guanine nucleotide-, isoproterenol-, and 
      fluoride-stimulated enzyme activity was significantly lower (P less than 0.05) in 
      the caudal artery of SHR as compared to WKY.
FAU - Bhalla, R C
AU  - Bhalla RC
FAU - Sharma, R V
AU  - Sharma RV
LA  - eng
GR  - HL 19027/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Blood Vessels
JT  - Blood vessels
JID - 0427130
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 9Y8NXQ24VQ (Propranolol)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Aorta/enzymology
MH  - Dose-Response Relationship, Drug
MH  - Guanosine Triphosphate/pharmacology
MH  - Hypertension/*enzymology
MH  - Isoproterenol/*pharmacology
MH  - Muscle, Smooth, Vascular/*enzymology
MH  - Propranolol/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sacrococcygeal Region/blood supply
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
PST - ppublish
SO  - Blood Vessels. 1982;19(3):109-16.