PMID- 7076855
OWN - NLM
STAT- MEDLINE
DCOM- 19820708
LR  - 20220310
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 69
IP  - 4
DP  - 1982 Apr
TI  - Mechanism of deoxycholic acid stimulation of the rabbit colon.
PG  - 985-92
AB  - Previous studies showed that deoxycholic acid (DCA) stimulated migrating action 
      potential complexes (MAPC) in the colon. The aim of this study was to clarify the 
      mechanism of DCA-stimulated colonic motility. Myoelectrical and contractile 
      activity were measured in New Zealand White rabbits from a loop constructed in 
      the proximal colon. During the control period, slow waves were present at a 
      frequency of 10.8 +/- 0.5 cycle/min and there were 1.5 +/- 0.5 MAPC/ h. After 
      adding DCA (16 mM) to the loop the slow wave activity was unchanged. However, 
      MAPC increased to 15.1 +/- 2.4 MAPC/h (P less than 0.001). MAPC activity was not 
      stimulated in the colonic smooth muscle outside the loop. The intraluminal 
      addition of procaine or tetrodotoxin to the colonic loop inhibited the 
      DCA-stimulated increase in MAPC activity (0.2 +/- 0.2 MAPC/h) (P less than 
      0.005). Intravenous administration of atropine or phentolamine also inhibited 
      MAPC activity that had been stimulated by DCA (P less than 0.005). Pretreatment 
      with 6-hydroxydopamine also inhibited an increase in MAPC activity. Propranolol, 
      trimethaphan camsylate, or hexamethonium had no effect on DCA stimulation of MAPC 
      activity. Although the concentration of bile salt increased in the mesenteric 
      venous outflow from the colonic loop, the intravenous administration of bile salt 
      did not stimulate colonic MAPC activity. These studies suggest: (a) the action of 
      DCA on smooth muscle activity is a local phenomenon, (b) the increase in MAPC 
      activity is dependent on intact cholinergic and alpha adrenergic neurons, and (c) 
      an increase in the concentration of bile salts in the serum is not associated 
      with an increase in colonic MAPC activity.
FAU - Shiff, S J
AU  - Shiff SJ
FAU - Soloway, R D
AU  - Soloway RD
FAU - Snape, W J Jr
AU  - Snape WJ Jr
LA  - eng
GR  - AM-25396/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 005990WHZZ (Deoxycholic Acid)
SB  - IM
MH  - Action Potentials/*drug effects
MH  - Animals
MH  - Colon/*physiology
MH  - Deoxycholic Acid/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Gastrointestinal Motility/*drug effects
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Rabbits
PMC - PMC370153
EDAT- 1982/04/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1982/04/01
CRDT- 1982/04/01 00:00
PHST- 1982/04/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1982/04/01 00:00 [entrez]
PHST- 1982/04/01 00:00 [pmc-release]
AID - 10.1172/jci110538 [doi]
PST - ppublish
SO  - J Clin Invest. 1982 Apr;69(4):985-92. doi: 10.1172/jci110538.