PMID- 70786
OWN - NLM
STAT- MEDLINE
DCOM- 19771014
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 74
IP  - 7
DP  - 1977 Jul
TI  - Dimeric immunoglobulin E serves as a unit signal for mast cell degranulation.
PG  - 2993-7
AB  - Rat immunoglobulin E (IgE) was treated with a crosslinking reagent, dimethyl 
      suberimidate, and fractionated by gel filtration into monomers, dimers, trimers, 
      and higher polymers. The fractions retained substantial ability to bind 
      specifically to mast cells. About one-third of the cell-bound dimers appeared to 
      bind bivalently. The fractions were assayed in vivo by passive cutaneous 
      anaphylaxis in rats, and for histamine or serotonin release in vitro using normal 
      or tumor mouse mast cells. The monomers showed no activity, while the dimers and 
      higher polymers gave excellent and approximately equivalent responses. We 
      conclude that IgE that has been crosslinked to form dimers prior to the addition 
      to mast cells can serve as a unit signal for triggering IgE-mediated exocytosis.
FAU - Segal, D M
AU  - Segal DM
FAU - Taurog, J D
AU  - Taurog JD
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 29878-26-0 (Dimethyl Suberimidate)
RN  - 333DO1RDJY (Serotonin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Ascitic Fluid/immunology
MH  - Basophils/*immunology
MH  - Dimethyl Suberimidate
MH  - *Histamine Release
MH  - *Immunoglobulin E/metabolism
MH  - Leukemia, Experimental/immunology
MH  - Mast Cells/*immunology
MH  - Mast-Cell Sarcoma/immunology
MH  - Molecular Weight
MH  - Passive Cutaneous Anaphylaxis
MH  - Protein Binding
MH  - Rats
MH  - Sarcoma, Experimental/immunology
MH  - Serotonin/metabolism
MH  - Structure-Activity Relationship
PMC - PMC431378
EDAT- 1977/07/01 00:00
MHDA- 1977/07/01 00:01
PMCR- 1978/01/01
CRDT- 1977/07/01 00:00
PHST- 1977/07/01 00:00 [pubmed]
PHST- 1977/07/01 00:01 [medline]
PHST- 1977/07/01 00:00 [entrez]
PHST- 1978/01/01 00:00 [pmc-release]
AID - 10.1073/pnas.74.7.2993 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1977 Jul;74(7):2993-7. doi: 10.1073/pnas.74.7.2993.