PMID- 7081454
OWN - NLM
STAT- MEDLINE
DCOM- 19820708
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 242
IP  - 5
DP  - 1982 May
TI  - Prolonged myocardial nucleotide depletion after brief ischemia in the open-chest 
      dog.
PG  - H818-26
AB  - Purine and pyrimidine nucleotides are essential energy sources for basic 
      metabolic reactions and play important roles in protein, glycogen, and nucleic 
      acid synthesis, cyclic nucleotide metabolism, and energy transfer reactions. 
      Brief coronary occlusions (12 min) were produced in seven open-chest dogs, and 
      repetitive myocardial samples were taken in order to determine the response of 
      the nucleotide pool to ischemia and reperfusion. During ischemia adenosine 
      5'-triphosphate (ATP) decreased to 57% of control, and similar decreases occurred 
      in the guanosine 5'-triphosphate (GTP), cytidine 5'-triphosphate (CTP), uridine 
      5'-triphosphate (UTP), and nicotinamide adenine dinucleotide (NAD+) pools. The 
      decrease in nucleotides was accompanied by an increase in nucleosides and bases. 
      After 60 min of reperfusion the content of all nucleotides had increased but was 
      still significantly less than nonischemic values. The content of nucleosides and 
      bases decreased immediately upon reperfusion. In contrast, creatine phosphate 
      (CP) fell to 10% of control during ischemia but rebounded to above control values 
      immediately upon reperfusion. Thus depletion of all nucleotide pools occurs 
      during ischemia, and with reperfusion nucleotide content is restored only slowly. 
      Delayed repletion is not caused by a defect in mitochondrial synthesis of ATP 
      because CP content is restored rapidly. The slow repletion of nucleotides may be 
      secondary to loss of nucleotide precursors during reperfusion and may result in 
      widespread alterations in myocardial metabolism.
FAU - Swain, J L
AU  - Swain JL
FAU - Sabina, R L
AU  - Sabina RL
FAU - McHale, P A
AU  - McHale PA
FAU - Greenfield, J C Jr
AU  - Greenfield JC Jr
FAU - Holmes, E W
AU  - Holmes EW
LA  - eng
GR  - HL-18468/HL/NHLBI NIH HHS/United States
GR  - R01 AM-12413/AM/NIADDK NIH HHS/United States
GR  - R23 HL-26831/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Nucleotides)
SB  - IM
MH  - Animals
MH  - Coronary Circulation
MH  - Dogs
MH  - Hemodynamics
MH  - Ischemia/*metabolism
MH  - Myocardium/*metabolism
MH  - Nucleotides/*metabolism
MH  - Perfusion
MH  - Reference Values
MH  - Time Factors
EDAT- 1982/05/11 19:15
MHDA- 1982/05/11 19:16
CRDT- 1982/05/11 19:15
PHST- 1982/05/11 19:15 [pubmed]
PHST- 1982/05/11 19:16 [medline]
PHST- 1982/05/11 19:15 [entrez]
AID - 10.1152/ajpheart.1982.242.5.H818 [doi]
PST - ppublish
SO  - Am J Physiol. 1982 May;242(5):H818-26. doi: 10.1152/ajpheart.1982.242.5.H818.