PMID- 7085344
OWN - NLM
STAT- MEDLINE
DCOM- 19820807
LR  - 20190830
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 74
IP  - 1
DP  - 1982
TI  - [The fluorescent staining of mitochondria in living HeLa- and LM-cells with new 
      acridine dyes (author's transl)].
PG  - 1-7
AB  - The fluorescent staining of mitochondria in living cells with new acridine dyes 
      is reported. The fluorescent dyes used are derivatives of acridine orange (AO) 
      and of 3-amino-6-methoxyacridine (AMA) with various residues in 9- or 10-position 
      (Scheme 1). They are either permanent cationic dyes or cations which are formed 
      by protonation in the culture medium. HeLa cells and mouse fibroblasts (LM cells) 
      have been used for our staining experiments. On favourable conditions we 
      succeeded in staining the mitochondria not only orthochromatically but also 
      metachromatically. Photodynamical effects which have been observed during the 
      exposure of the stained cells in the fluorescence microscope are described. The 
      residues in 9- or 10-position favour the dye accumulation in the mitochondria. 
      Vital staining with the basic compounds AO and AMA however leads to the formation 
      of metachromatically stained lysosomes in the orthochromatically stained 
      cytoplasm. The dye 3-amino-6-methoxy-9-(2-hydroxyethyl)acridine stains the 
      nucleus of living cells.
FAU - Erbrich, U
AU  - Erbrich U
FAU - Naujok, A
AU  - Naujok A
FAU - Petschel, K
AU  - Petschel K
FAU - Zimmermann, H W
AU  - Zimmermann HW
LA  - ger
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Uber die Vitalfluorochromierung von Mitochondrien in HeLa- und LM-Zellen mit 
      neuen Acridinfarbstoffen.
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - 0 (Aminoacridines)
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - *Aminoacridines
MH  - Animals
MH  - Cells, Cultured
MH  - Fibroblasts/ultrastructure
MH  - *Fluorescent Dyes
MH  - HeLa Cells/ultrastructure
MH  - Humans
MH  - Mice
MH  - Mitochondria/*ultrastructure
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1007/BF00495046 [doi]
PST - ppublish
SO  - Histochemistry. 1982;74(1):1-7. doi: 10.1007/BF00495046.