PMID- 7106191
OWN - NLM
STAT- MEDLINE
DCOM- 19821029
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 80
IP  - 4
DP  - 1982 Jun 4
TI  - Response of the guinea-pig urinary bladder to purine and pyrimidine nucleotides.
PG  - 401-6
AB  - The ability of purines and pyrimidines to cause and inhibit contractile responses 
      was examined in strips of guinea-pig urinary bladder. Adenosine 5'-triphosphate 
      (ATP), beta, gamma-methylene ATP (APPCP), adenosine 5'-diphosphate (ADP), 
      guanosine 5'-triphosphate (GTP) and cytidine 5'-triphosphate (CTP) caused 
      concentration-dependent contractions of the bladder. The order of potency was 
      APPCP greater than ATP greater than GTP=CTP greater than ADP. Adenosine 
      5'-monophosphate, adenosine, guanosine 5'-diphosphate, guanosine 
      5'-monophosphate, guanosine, cytidine 5'-diphosphate, cytidine 5'-monophosphate, 
      adenosine, guanosine 5'-diphosphate, guanosine 5'-monophosphate, guanosine, 
      cytidine 5'-diphosphate, cytidine 5'-monophosphate and cytidine had no apparent 
      contractile activity up to 10(-3) M. Cumulative administrations of 5 X 10(-4) M 
      APPCP, ATP, ADP, GTP or CTP resulted in complex desensitization of the tissue to 
      the contractile activity of the nucleotide. Tissues desensitized to GTP or CTP 
      were non-responsive to ATP suggesting cross-tachyphylaxis. Nucleotides lacking 
      contractile activity or nucleosides did not alter the response of the bladder to 
      ATP; except AMP and adenosine which significantly reduced the contraction. These 
      results suggest that the polyphosphate structure, and not the base, is the 
      determining portion of the nucleotide for promoting contractile activity and the 
      development of tachyphylaxis.
FAU - Lukacsko, P
AU  - Lukacsko P
FAU - Krell, R D
AU  - Krell RD
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Purine Nucleotides)
RN  - 0 (Pyrimidine Nucleotides)
SB  - IM
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Purine Nucleotides/*pharmacology
MH  - Pyrimidine Nucleotides/*pharmacology
MH  - Structure-Activity Relationship
MH  - Tachyphylaxis
MH  - Urinary Bladder/*drug effects
EDAT- 1982/06/04 00:00
MHDA- 1982/06/04 00:01
CRDT- 1982/06/04 00:00
PHST- 1982/06/04 00:00 [pubmed]
PHST- 1982/06/04 00:01 [medline]
PHST- 1982/06/04 00:00 [entrez]
AID - 0014-2999(82)90086-3 [pii]
AID - 10.1016/0014-2999(82)90086-3 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1982 Jun 4;80(4):401-6. doi: 10.1016/0014-2999(82)90086-3.