PMID- 7108445
OWN - NLM
STAT- MEDLINE
DCOM- 19821012
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 156
IP  - 3
DP  - 1982 Sep 1
TI  - Deregulation of idiotype expression. Induction of tolerance in an anti-idiotypic 
      response.
PG  - 898-911
AB  - The induction of tolerance in an anti-idiotypic response was attempted by in vivo 
      exposure to excess idiotype. Monoclonal immunoglobulin from the 
      anti-p-azobenzenearsonate (ABA) hybridoma R16.7 was used as a representative of 
      cross-reactive idiotype-positive (CRI+) antibodies because this hybridoma protein 
      (HP) shares one or more closely related public idiotypic determinants with the 
      serum CRI in A/J mice. Immunologic unresponsiveness was established by a single 
      injection of the R16.7 idiotype and persisted for at least 6 wk. The level of 
      circulating anti-idiotypic antibodies in tolerized A/J mice was significantly 
      depressed after immunogenic challenge with eigher antigen, ABA-keyhole limpet 
      hemocyanin (KLH), or the idiotype R16.7 HP. Experimental depletion of 
      anti-idiotypic antibodies by tolerization allowed assessment of immunoregulation 
      within this altered idiotype-anti-idiotype network. Deregulation of idiotype 
      expression in tolerized mice challenged with ABA-KLH was manifest with up to 96% 
      of the anti-ABA antibodies cross-reacting with the R16.7 idiotype. This selective 
      enhancement of a major idiotype was accomplished without substantial alteration 
      of the level of the overall anti-hapten response. Both the unresponsiveness 
      established in anti-idiotypic antibody-producing cells and the enhanced synthesis 
      in idiotype-producing cells were stable upon adoptive transfer into lethally 
      irradiated, syngeneic recipients. Finally, previous immunization with the antigen 
      ABA-KLH interfered with the induction of unresponsiveness to the idiotype. This 
      interference is presumed to be mediated by prior activation of anti-idiotypic 
      cells and/or antibody because injection of antigen with tolerogenic idiotype did 
      not abrogate tolerance induction.
FAU - Ortiz-Ortiz, L
AU  - Ortiz-Ortiz L
FAU - Weigle, W O
AU  - Weigle WO
FAU - Parks, D E
AU  - Parks DE
LA  - eng
GR  - AG01629/AG/NIA NIH HHS/United States
GR  - AI07007/AI/NIAID NIH HHS/United States
GR  - RRO-5514/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 7334-23-8 (p-Azobenzenearsonate)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - *Immune Tolerance
MH  - Immunization
MH  - Immunization, Passive
MH  - Immunization, Secondary
MH  - Immunoglobulin Idiotypes/analysis/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred A
MH  - p-Azobenzenearsonate/immunology
PMC - PMC2186787
EDAT- 1982/09/01 00:00
MHDA- 1982/09/01 00:01
PMCR- 1983/03/01
CRDT- 1982/09/01 00:00
PHST- 1982/09/01 00:00 [pubmed]
PHST- 1982/09/01 00:01 [medline]
PHST- 1982/09/01 00:00 [entrez]
PHST- 1983/03/01 00:00 [pmc-release]
AID - 82267762 [pii]
AID - 10.1084/jem.156.3.898 [doi]
PST - ppublish
SO  - J Exp Med. 1982 Sep 1;156(3):898-911. doi: 10.1084/jem.156.3.898.