PMID- 7113699
OWN - NLM
STAT- MEDLINE
DCOM- 19821012
LR  - 20190904
IS  - 0001-6632 (Print)
IS  - 0001-6632 (Linking)
VI  - 32
IP  - 4
DP  - 1982 Jul
TI  - DNA template activity in rat brain tumors induced by transplacental 
      administration of ethylnitrosourea.
PG  - 595-603
AB  - The ultracytochemical acridine orange (AO) method has been employed to 
      demonstrate DNA template activity within experimental brain tumors induced by 
      transplacental administration ethylnitrosourea (ENU) in Sprague-Dawley rats. 
      Ultrastructural examination revealed that acridine orange binds to DNA 
      exclusively within the active extended euchromatin portion of the cell nucleus of 
      brain tumor and of foci of atypical cell proliferation. No AO reaction products 
      were visible in the non-neoplastic cell nuclei adjacent to the brain tumors. The 
      percentages of AO positive cells in brain tumor cells in rats given an 
      intraperitoneal injection of 3H-thymidine 1 hr before sacrifice were between 2.4% 
      and 8.0%. The average number of AO chromatin reaction products per single section 
      of a cell nucleus varied between 7 and 21. The ratios of areas of euchromatin to 
      heterochromatin were found to be slightly larger in AO positive nuclei then in AO 
      negative nuclei in each of the tumors. The present results suggested that rat 
      brain tumors induced by transplacental administration ENU exhibit de-repression 
      of DNA template normally repressed in the adult state. The usefulness of this 
      method for study on the development of experimental brain tumors is briefly 
      discussed.
FAU - Hara, H
AU  - Hara H
FAU - Moriki, T
AU  - Moriki T
FAU - Miyao, M
AU  - Miyao M
FAU - Enzan, H
AU  - Enzan H
FAU - Kutsukake, F
AU  - Kutsukake F
FAU - Yamane, T
AU  - Yamane T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Acta Pathol Jpn
JT  - Acta pathologica japonica
JID - 0372637
RN  - 0 (DNA, Neoplasm)
RN  - F30N4O6XVV (Acridine Orange)
RN  - P8M1T4190R (Ethylnitrosourea)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Brain Neoplasms/*analysis/chemically induced/ultrastructure
MH  - DNA, Neoplasm/*analysis
MH  - Ethylnitrosourea
MH  - Female
MH  - Maternal-Fetal Exchange
MH  - Neoplasms, Experimental/analysis/chemically induced/ultrastructure
MH  - Oligodendroglioma/*analysis/chemically induced/ultrastructure
MH  - Placenta
MH  - Pregnancy
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Templates, Genetic
EDAT- 1982/07/01 00:00
MHDA- 1982/07/01 00:01
CRDT- 1982/07/01 00:00
PHST- 1982/07/01 00:00 [pubmed]
PHST- 1982/07/01 00:01 [medline]
PHST- 1982/07/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1982.tb02060.x [doi]
PST - ppublish
SO  - Acta Pathol Jpn. 1982 Jul;32(4):595-603. doi: 10.1111/j.1440-1827.1982.tb02060.x.