PMID- 7127284
OWN - NLM
STAT- MEDLINE
DCOM- 19821216
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 42
IP  - 11
DP  - 1982 Nov
TI  - Characterization of protein carboxyl-O-methyltransferase in the spontaneous in 
      vivo murine C-1300 neuroblastoma.
PG  - 4433-6
AB  - Protein carboxy-O-methyltransferase (PCM) activity was determined in subcellular 
      fractions prepared from C-1300 neuroblastoma tumors following transplantation and 
      growth in male A/J mice. Fractions were obtained by differential centrifugation, 
      and PCM activity was determined in all fractions in the presence (+gel) and 
      absence (-gel) of an exogenous substrate, gelatin. Sixty % of the PCM activity in 
      the absence of exogenous substrate (-gel) was contained in the crude 800 X g 
      particulate fraction, whereas 80% of the PCM activity in the presence of gelatin 
      (+gel) was present in the postmicrosomal (100,000 X g) supernatant. The latter 
      fraction also contained the highest specific activity of PCM. A Km of 3.2 X 
      10(-6) M and a Vmax of 5.3 pmol per mg protein per min were obtained for PCM 
      activity (+gel) in the high-speed supernatant. Cytoplasmic PCM was highly 
      sensitive to competitive inhibition by S-adenosylhomocysteine and the 
      S-adenosyl-homocysteine analogs sinefungin and A-9145C with Ki values of 0.64, 
      0.47, and 0.05 microM, respectively. These data demonstrate that PCM present in 
      murine neuroblastoma has characteristics similar to those of PCM isolated from 
      other adrenergic and neuronal tissues. S-Adenosyl-homocysteine analogs may be 
      useful probes for studying the role of PCM as a modulator of cell function in 
      neurogenic and neoplastic tissues.
FAU - O'Dea, R F
AU  - O'Dea RF
FAU - Pons, G
AU  - Pons G
FAU - Hansen, J A
AU  - Hansen JA
FAU - Mirkin, B L
AU  - Mirkin BL
LA  - eng
GR  - HL-00565/HL/NHLBI NIH HHS/United States
GR  - NS-17194/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - EC 2.1.1.- (Protein Methyltransferases)
RN  - EC 2.1.1.- (Protein O-Methyltransferase)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred A
MH  - Neoplasms, Experimental/enzymology
MH  - Neuroblastoma/*enzymology
MH  - Protein Methyltransferases/*metabolism
MH  - Protein O-Methyltransferase/isolation & purification/*metabolism
MH  - Subcellular Fractions/enzymology
EDAT- 1982/11/01 00:00
MHDA- 1982/11/01 00:01
CRDT- 1982/11/01 00:00
PHST- 1982/11/01 00:00 [pubmed]
PHST- 1982/11/01 00:01 [medline]
PHST- 1982/11/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1982 Nov;42(11):4433-6.