PMID- 7139620
OWN - NLM
STAT- MEDLINE
DCOM- 19830119
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 42
IP  - 12
DP  - 1982 Dec
TI  - Combined actions of 5-fluorouracil and 
      1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea on human colonic carcinoma 
      cells in vitro.
PG  - 5172-5
AB  - Two lines of human colonic carcinoma cells have different sensitivities to 
      5-fluorouracil and 1-(2-chlorethyl)-3-(4-methylcyclohexyl)-1-nitrosourea 
      (methyl-CCNU). The growth of the BE line is 50% inhibited by 330 microM 
      5-fluorouracil and 15 microM methyl-CCNU, and the HT-29 line is 50% inhibited by 
      85 and 140 microM, respectively. On cloning, 50% of BE cells are killed by 500 
      microM 5-fluorouracil and 6 microM methyl-CCNU, and the HT-29 cells are killed by 
      250 and 120 microM respectively. When the drugs were combined, there were 
      additive effects which occurred in cell growth of both lines and killing of BE 
      cells. Synergism occurred in killing HT-29 cells when a low concentration of 
      5-fluorouracil was combined with methyl-CCNU. The synergism did not increase with 
      increasing concentrations of 5-fluorouracil. Both drugs caused growth delay of 
      spheroids (HT-29 cells), an effect that was additive when the drugs were 
      combined. Growth inhibition of both lines in monolayer culture by 5-fluorouracil 
      was more sensitive than was cell killing or inhibition of spheroid growth but, 
      with methyl-CCNU, killing of BE cells was more sensitive than was growth 
      inhibition. HT-29 cells showed similar sensitivity to methyl-CCNU in all three 
      systems. The type and sensitivity of drug effect seen in vitro depends on the 
      particular drug used, the cell line tested, and the parameter measured.
FAU - Sutton, J E
AU  - Sutton JE
FAU - Roos, I A
AU  - Roos IA
FAU - Hillcoat, B L
AU  - Hillcoat BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Nitrosourea Compounds)
RN  - 13909-09-6 (Semustine)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Colonic Neoplasms/*drug therapy
MH  - Drug Evaluation, Preclinical
MH  - Drug Therapy, Combination
MH  - Fluorouracil/*therapeutic use
MH  - Humans
MH  - Kinetics
MH  - Nitrosourea Compounds/*therapeutic use
MH  - Semustine/*therapeutic use
EDAT- 1982/12/01 00:00
MHDA- 1982/12/01 00:01
CRDT- 1982/12/01 00:00
PHST- 1982/12/01 00:00 [pubmed]
PHST- 1982/12/01 00:01 [medline]
PHST- 1982/12/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1982 Dec;42(12):5172-5.