PMID- 714078
OWN - NLM
STAT- MEDLINE
DCOM- 19790115
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 58
IP  - 1
DP  - 1978 Sep
TI  - The effect of the mode of administration of nitrogen mustard and cytosine 
      arabinoside on the production of chromosomal aberrations in mouse bone marrow and 
      ascites tumour cells.
PG  - 67-77
AB  - The cytogenetic effects of intraperitoneally (i.p.) and subcutaneously (s.c.) 
      administered nitrogen mustard (HN2) and cytosine arabinoside (ara-C) on 
      bone-marrow and ascites tumour cells of mice were studied. Ehrlich ascites 
      tumour-bearing mice were treated with the mutagens, and cytological preparations 
      were made from ascites tumour and bone-marrow cells of the same animal. The 
      following parameters were investigated: frequencies of mitotic and chromosomal 
      aberrations, time of aberration maxima and aberration spectra. HN2 (0.68 mg/kg 
      b.w.), when given i.p., induced in ascites tumour cells a strong inhibition of 
      mitotic frequency and very high aberration rates, whereas in bone marrow no 
      aberrant chromosomes were observed. On the other hand, after s.c. administration, 
      the same dose induced more aberrant metaphases in bone marrow than in tumour 
      cells. Ara-C (315 mg/kg b.w.) resulted, after s.c. administration, in higher 
      aberration frequencies both in ascites and bone-marrow cells compared with i.p. 
      treatment. All ascites tumour cells showed higher aberration requencies than 
      bone-marrow cells. In bone marrow the aberration maximum occurred as soon as 6 h 
      after treatment. Furthermore, clear differences with respect ot the types of 
      aberration found in the two systems were evident. The differences caused by the 
      different modes of administration in two different types of cell are discussed in 
      terms of metabolic inactivation and differences of the two tissues with respect 
      to karyotype, cell cycle time and repair capacity.
FAU - Wobus, A M
AU  - Wobus AM
FAU - Schoneich, J
AU  - Schoneich J
FAU - Thieme, R
AU  - Thieme R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 04079A1RDZ (Cytarabine)
RN  - 50D9XSG0VR (Mechlorethamine)
SB  - IM
MH  - Animals
MH  - Bone Marrow/drug effects/ultrastructure
MH  - Carcinoma, Ehrlich Tumor/*ultrastructure
MH  - *Chromosome Aberrations
MH  - Chromosomes/drug effects
MH  - Cytarabine/*administration & dosage
MH  - Female
MH  - Injections, Intraperitoneal
MH  - Injections, Subcutaneous
MH  - Mechlorethamine/*administration & dosage
MH  - Mice
MH  - Mice, Inbred Strains
EDAT- 1978/09/01 00:00
MHDA- 1978/09/01 00:01
CRDT- 1978/09/01 00:00
PHST- 1978/09/01 00:00 [pubmed]
PHST- 1978/09/01 00:01 [medline]
PHST- 1978/09/01 00:00 [entrez]
AID - 0165-1218(78)90096-4 [pii]
AID - 10.1016/0165-1218(78)90096-4 [doi]
PST - ppublish
SO  - Mutat Res. 1978 Sep;58(1):67-77. doi: 10.1016/0165-1218(78)90096-4.