PMID- 7199295
OWN - NLM
STAT- MEDLINE
DCOM- 19820412
LR  - 20131121
IS  - 0004-4172 (Print)
IS  - 0004-4172 (Linking)
VI  - 31
IP  - 12
DP  - 1981
TI  - Comparative determinations of 5-fluorouracil in plasma using GC/MS and HPLC.
PG  - 2048-53
AB  - Gas chromatography-mass spectrometry (GC/MS) and high performance liquid 
      chromatography (HPLC) procedures have developed to determine 5-fluorouracil 
      (5-FU) in human plasmas. Internal standard in both systems was 5-bromouracil 
      (5-BrU). The HPLC assay used an extracted plasma with an ether-isopropanol 
      mixture (75-25): the analyses were performed on a mu Porasil column (10 
      micrometer) with hexane-ethanol (75-25) as elution solvent (detection limit 50 
      ng/ml). For GC/MS, after precipitation of proteins, the plasma was purified 
      through a microcolumn packed with AG1 X 4 resin, and then the components were 
      methylated. Electron impact (EI) and selective ion monitoring (SIM) mode was 
      retained for GC/MS quantitative studies (detection limit 5 ng/ml). Moreover, the 
      authors compared these two techniques by measurements on plasma samples obtained 
      from patients after rapid i.v. injections, infusions and p.o. administration.
FAU - Aubert, C
AU  - Aubert C
FAU - Luccioni, C
AU  - Luccioni C
FAU - Coassolo, P
AU  - Coassolo P
FAU - Sommadossi, J P
AU  - Sommadossi JP
FAU - Cano, J P
AU  - Cano JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Arzneimittelforschung
JT  - Arzneimittel-Forschung
JID - 0372660
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Administration, Oral
MH  - Chromatography, High Pressure Liquid
MH  - Fluorouracil/administration & dosage/*blood
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Injections, Intravenous
EDAT- 1981/01/01 00:00
MHDA- 1981/01/01 00:01
CRDT- 1981/01/01 00:00
PHST- 1981/01/01 00:00 [pubmed]
PHST- 1981/01/01 00:01 [medline]
PHST- 1981/01/01 00:00 [entrez]
PST - ppublish
SO  - Arzneimittelforschung. 1981;31(12):2048-53.