PMID- 7201778
OWN - NLM
STAT- MEDLINE
DCOM- 19820917
LR  - 20190920
IS  - 0166-3542 (Print)
IS  - 0166-3542 (Linking)
VI  - 2
IP  - 1-2
DP  - 1982 May
TI  - Antiviral effects if ribavirin and 6-mercapto-9-tetrahydro-2-furylpurine against 
      dengue viruses in vitro.
PG  - 69-79
AB  - The antiviral effects of ribavirin 
      (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide) and 
      6-mercapto-9-tetrahydro-2-furylpurine (6-MPTF) against dengue viruses were 
      examined in vitro. Ribavirin significantly reduced the growth of dengue virus 
      types 1-4 in LLC-MK2 cells at concentrations well below cytotoxic levels (cell 
      viability was determined by trypan blue dye exclusion) Addition of guanosine to 
      ribavirin-treated dengue virus-infected cell cultures completely reversed the 
      antiviral effect of the drug. In contrast, ribavirin had no effect on dengue 
      virus replication in human peripheral blood leukocytes (PBL). 6-MPIF, a specific 
      inhibitor of hypoxanthine-guanine phosphoribosyltransferase, did not 
      significantly reduce the growth of dengue viruses in either LIC-MK2 cells or 
      human PBL. However, synergistic effects of 6-MPTF and ribavirin were observed, as 
      combined treatment of the drugs markedly suppressed the replication of dengue 
      viruses in human PBL. The successful demonstration that dengue virus replication 
      in mononuclear leukocytes is markedly suppressed by the combined treatment of 
      ribavirin and 6-MPTF signals a need to evaluate the efficacy of this treatment 
      against dengue virus infections in vivo.
FAU - Koff, W C
AU  - Koff WC
FAU - Elm, J L Jr
AU  - Elm JL Jr
FAU - Halstead, S B
AU  - Halstead SB
LA  - eng
GR  - 2-R01-HD08693/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (6-mercapto-9-(tetrahydro-2-furyl)purine)
RN  - 0 (Nucleosides)
RN  - 0 (Ribonucleosides)
RN  - 49717AWG6K (Ribavirin)
RN  - E7WED276I5 (Mercaptopurine)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dengue Virus/*drug effects/growth & development
MH  - Drug Synergism
MH  - Fibroblasts
MH  - Humans
MH  - Leukocytes/drug effects
MH  - Macaca mulatta
MH  - Mercaptopurine/*analogs & derivatives/pharmacology
MH  - Nucleosides/pharmacology
MH  - Ribavirin/*pharmacology
MH  - Ribonucleosides/*pharmacology
EDAT- 1982/05/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1982/05/01 00:00
PHST- 1982/05/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1982/05/01 00:00 [entrez]
AID - 0166-3542(82)90027-4 [pii]
AID - 10.1016/0166-3542(82)90027-4 [doi]
PST - ppublish
SO  - Antiviral Res. 1982 May;2(1-2):69-79. doi: 10.1016/0166-3542(82)90027-4.