PMID- 7223772 OWN - NLM STAT- MEDLINE DCOM- 19810613 LR - 20061115 IS - 0002-9335 (Print) IS - 0002-9335 (Linking) VI - 47 IP - 4 DP - 1981 Apr TI - Free coagulase and thermostable nuclease reactions of staphylococci: a comparison. PG - 237-9 AB - Two-hundred-fifty-five staphylococcal isolates (clinical) were tested for both free coagulase (CGase) and thermostable nuclease (TNase) production. Two-hundred-ten of the 255 isolates were identified as Staphylococcus aureus when both strongly positive TNase and CGase reactions (human and rabbit plasma) were observed with these strains. Forty-five of the 255 isolates were identified as Staphylococcus species, other than S. aureus when either weakly positive TNase and CGase (rabbit plasma) reactions or negative TNase and CGase (rabbit and human plasma) reactions were observed. This experiment suggest that the identification of staphylococcal isolates as S. aureus can be based upon demonstration of both strongly positive TNase and CGase reactions. FAU - Sukroongreung, S AU - Sukroongreung S FAU - Nilakul, C AU - Nilakul C FAU - Sirinanunta, S AU - Sirinanunta S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Am J Med Technol JT - The American journal of medical technology JID - 0370505 RN - 0 (Coagulase) RN - EC 3.1.31.1 (Micrococcal Nuclease) SB - IM MH - Coagulase/*analysis MH - Humans MH - Micrococcal Nuclease/*analysis MH - Staphylococcus aureus/enzymology/*isolation & purification EDAT- 1981/04/01 00:00 MHDA- 1981/04/01 00:01 CRDT- 1981/04/01 00:00 PHST- 1981/04/01 00:00 [pubmed] PHST- 1981/04/01 00:01 [medline] PHST- 1981/04/01 00:00 [entrez] PST - ppublish SO - Am J Med Technol. 1981 Apr;47(4):237-9.