PMID- 7225331
OWN - NLM
STAT- MEDLINE
DCOM- 19810720
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 20
IP  - 5
DP  - 1981 Mar 3
TI  - In vitro microtubule assembly regulation by divalent cations and nucleotides.
PG  - 1318-22
AB  - Mg2+ and guanosine 5'-triphosphate (GTP) are usually used to assemble microtubule 
      protein (tubulin + microtubule-associated proteins) or tubulin into microtubules 
      in vitro. Recently, it has been shown that Mn2+ will substitute for Mg2+ in 
      inducing pure tubulin + GTP to assemble into microtubules. We find that Mn2+ also 
      substitute for Mg2+ in inducing two-cycle calf microtubule protein (MTP) + GTP to 
      assemble into microtubules. Zn2+ or Co2+ induces MTP + GTP to form sheets with 
      more than 13 protofilaments. We find that Co2+ also substitutes for Zn2+ in 
      inducing tubulin to form 200-nm tubules similar to those reported with Zn2+. To 
      learn whether metal-induced assembly is due to direct binding and/or a metal-GTP 
      complex, metal-induced assembly of MTP and tubulin was studied in the presence of 
      CrIIIGTP or taxol, two probes which promote assembly in the absence of added GTP. 
      With CrGTP, Mg2+ and ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic 
      acid (EGTA) were required for optimal assembly into microtubules, and Mn2+ could 
      substitute for Mg2+. MTP incubated with Zn2+ and CrGTP assembled into sheets, 
      shorter than but similar to those induced by Zn2+ + GTP. Mg2+-induced 
      microtubules and Zn2+-induced sheets contained 0.45 mol of [8-3H]GTP/mol of 
      tubulin if assembled from [8-3H]GTP and a mixture of 0.25 mol of [8-3H]CrGTP, 0.1 
      mol of [8-3H]GDP, and 0.05 mol of [8-3H]CrGDP/mol of tubulin if assembled from 
      [8-3H]CrGTP. Zn2+ induced taxol-treated MTP to form sheets. Sheets were also 
      induced from tubulin + Zn2+ and either CrGTP or taxol. These studies suggest that 
      the Zn2+-induced structures are not due to a Zn-GTP complex and that Mg2+ does 
      not promote assembly only through a Mg-GTP complex.
FAU - Gaskin, F
AU  - Gaskin F
LA  - eng
GR  - AG00002/AG/NIA NIH HHS/United States
GR  - NS 12418/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Cations, Divalent)
RN  - 0 (Tubulin)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - I38ZP9992A (Magnesium)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism/ultrastructure
MH  - Cations, Divalent
MH  - Cattle
MH  - Guanosine Triphosphate/*pharmacology
MH  - Kinetics
MH  - Magnesium/pharmacology
MH  - Manganese/pharmacology
MH  - Microtubules/*ultrastructure
MH  - Tubulin/*metabolism
MH  - Zinc/pharmacology
EDAT- 1981/03/03 00:00
MHDA- 1981/03/03 00:01
CRDT- 1981/03/03 00:00
PHST- 1981/03/03 00:00 [pubmed]
PHST- 1981/03/03 00:01 [medline]
PHST- 1981/03/03 00:00 [entrez]
AID - 10.1021/bi00508a043 [doi]
PST - ppublish
SO  - Biochemistry. 1981 Mar 3;20(5):1318-22. doi: 10.1021/bi00508a043.