PMID- 7248171
OWN - NLM
STAT- MEDLINE
DCOM- 19810922
LR  - 20181113
IS  - 0007-1021 (Print)
IS  - 0007-1021 (Linking)
VI  - 62
IP  - 3
DP  - 1981 Jun
TI  - Tritiated-thymidine-induced increased DNA content and irreversible 
      differentiation in a human melanoma cell line.
PG  - 289-96
AB  - When cultured in the presence of 2.0 microCi/ml (methyl-3H)thymidine (3H-TdR) the 
      growth rate of 6 human melanoma lines and 1 subline progressively slowed then 
      stopped, a change that was accompanied by loss of reproductive viability as 
      assessed by colony formation in agar, but unaccompanied by a comparable 
      inhibition of thymidine incorporation. In all cases increases in cell size, 
      nuclear size and DNA content were observed. In 1 cell line only, MM96, these 
      changes were accompanied by a profound increase in morphological differentiation. 
      Despite this MM96 did not show increased differentiation in response to 2 x 
      10(-7) M alpha melanocyte stimulating-hormone (alpha MSH), which in fact 
      stimulated growth, or in response to 10(-3) M N6,O2'-dibutyryl adenosine 
      3':5'-monophosphate (db-cAMP), 10(-3) M theophylline or 5 x 10(-4)M 
      guanosine-5'-triphosphate (GTP), all of which retarded growth. With none of the 
      cell lines was differentiation increased in response to 10(-3)M db-cAMP although 
      in each case growth was retarded. These results reinforce the importance of 
      colony assays vs DNA synthesis studies in assessing reproductive viability and 
      show that supra-reproductively lethal levels of 3H-TdR can by-pass defects in the 
      differentiation pathway of at least one, but not all, human melanoma cell lines.
FAU - Sheridan, J W
AU  - Sheridan JW
FAU - Simmons, R J
AU  - Simmons RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Exp Pathol
JT  - British journal of experimental pathology
JID - 0372543
RN  - 0 (DNA, Neoplasm)
RN  - 958-74-7 (methylthymidine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Line
MH  - DNA, Neoplasm/*biosynthesis
MH  - Humans
MH  - Melanoma/*metabolism/pathology
MH  - Mitotic Index
MH  - Thymidine/analogs & derivatives/metabolism
MH  - Time Factors
PMC - PMC2041686
EDAT- 1981/06/01 00:00
MHDA- 1981/06/01 00:01
PMCR- 1981/06/01
CRDT- 1981/06/01 00:00
PHST- 1981/06/01 00:00 [pubmed]
PHST- 1981/06/01 00:01 [medline]
PHST- 1981/06/01 00:00 [entrez]
PHST- 1981/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Br J Exp Pathol. 1981 Jun;62(3):289-96.