PMID- 727720
OWN - NLM
STAT- MEDLINE
DCOM- 19790221
LR  - 20061115
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 3
IP  - 5
DP  - 1978 May
TI  - A technique for the elution of cell-surface antibody from human brain tissue.
PG  - 403-5
AB  - An artificial system is described in which anti-dinitrophenyl (DNP) antibody 
      attaches to hapten-conjugated human brain tissue. Treatment of this material with 
      acetic acid at pH 2.5 to 3.0 for 90 seconds followed by immediate neutralization 
      results in dissociation of antibody from the hapten-conjugated brain. Less than 1 
      ng of specific antibody can be detected, as determined by the ability of eluted 
      material to combine with DNP in an established radioimmunoassay system. Similar 
      methods may be applied to neurological disorders in which immunoglobulins are 
      thought to be produced in the central nervous system.
FAU - Gilden, D
AU  - Gilden D
FAU - Devlin, M
AU  - Devlin M
FAU - Wroblewska, Z
AU  - Wroblewska Z
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
RN  - 0 (Antibodies)
RN  - 0 (Dinitrophenols)
SB  - IM
MH  - Antibodies/*isolation & purification
MH  - Brain/*immunology
MH  - Dinitrophenols/immunology
MH  - Humans
MH  - Methods
MH  - Radioimmunoassay
EDAT- 1978/05/01 00:00
MHDA- 1978/05/01 00:01
CRDT- 1978/05/01 00:00
PHST- 1978/05/01 00:00 [pubmed]
PHST- 1978/05/01 00:01 [medline]
PHST- 1978/05/01 00:00 [entrez]
AID - 10.1002/ana.410030507 [doi]
PST - ppublish
SO  - Ann Neurol. 1978 May;3(5):403-5. doi: 10.1002/ana.410030507.