PMID- 7323414
OWN - NLM
STAT- MEDLINE
DCOM- 19820322
LR  - 20191031
IS  - 0378-5858 (Print)
IS  - 0378-5858 (Linking)
VI  - 3
IP  - 1-6
DP  - 1980
TI  - Human platelets and glomerular basal lamina interaction.
PG  - 205-11
AB  - Collagen is one of the major constituents of glomerular basal lamina. Its 
      thrombogenecity has been systematically studied in our laboratory by 
      aggregometry, adenine nucleotide release assay, and electron microscopy. The 
      purified human glomerular basal lamina (HGBL) preparation does not induce 
      platelet degranulation, nucleotide release, or aggregation, although adhesion and 
      spreading of platelets on HGBL are observed. Isolated monomeric HGBL collagen or 
      insoluble HGBL collagen in its native state of organization are similarly 
      inactive. Modification of carbohydrate moieties by sialase, alpha-glucosidase, or 
      sodium periodate oxidation has no effect on HGBL's inability to induce platelet 
      release reaction or aggregation. Therefore, HGBL collagen is not thrombogenic as 
      has been suspected. Adhesion and spreading of platelets on HGBL, which require 
      the noncollagen constituents of HGBL and divalent cations, represent a temporary 
      capillary pavement for endothelial defect distinct from thrombogenic activity of 
      platelets.
FAU - Huang, T W
AU  - Huang TW
FAU - Benditt, E P
AU  - Benditt EP
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Ren Physiol
JT  - Renal physiology
JID - 7901911
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Macromolecular Substances)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adenine Nucleotides/metabolism
MH  - Basement Membrane/*physiology
MH  - Blood Coagulation
MH  - Blood Platelets/*physiology
MH  - Collagen/analysis/*physiology
MH  - Humans
MH  - Kidney Glomerulus/*blood supply
MH  - Macromolecular Substances
MH  - Microscopy, Polarization
MH  - Platelet Adhesiveness
MH  - Platelet Aggregation
MH  - Research Design
EDAT- 1980/01/01 00:00
MHDA- 1980/01/01 00:01
CRDT- 1980/01/01 00:00
PHST- 1980/01/01 00:00 [pubmed]
PHST- 1980/01/01 00:01 [medline]
PHST- 1980/01/01 00:00 [entrez]
AID - 10.1159/000172762 [doi]
PST - ppublish
SO  - Ren Physiol. 1980;3(1-6):205-11. doi: 10.1159/000172762.