PMID- 7364940
OWN - NLM
STAT- MEDLINE
DCOM- 19800616
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 65
IP  - 5
DP  - 1980 May
TI  - Synergism of thyroid hormone and high carbohydrate diet in the induction of 
      lipogenic enzymes in the rat. Mechanisms and implications.
PG  - 1126-34
AB  - We have investigated the relationship between the administration of 
      triiodothyronine (T3) and a high carbohydrate (CHO) fat-free diet in the 
      induction of lipogenic enzymes in two rat tissues, liver, and fat. Male 
      thyroidectomized rats were treated with graded daily doses of T3 and either 
      supplemented with a high CHO diet or left on a regular diet. Enzymes studied 
      included malic enzyme (ME), fatty acid synthetase, glucose-6-phosphate 
      dehydrogenase, and 6-phosphogluconate dehydrogenase. In the liver, all four 
      lipogenic enzymes showed a synergistic response between T3 administration and 
      high CHO feeding. In fat, ME also responded synergistically. The interaction was 
      reflected in an increased sensitivity to T3. The dose of T3 required to achieve 
      50% maximal response was reduced three- to seven-fold by the high CHO diet. This 
      phenomenon could not be attributed to a dietary-induced alteration either in T3 
      metabolism or in number or affinity of the T3-nuclear receptors. Moreover, 
      studies of the relative rate of synthesis of ME suggested a simultaneous time of 
      onset in the induction of ME, within 2 h after the application of either T3 or 
      CHO. Thus, it is unlikely that either stimulus is secondary to the other. Since 
      parallel experiments from this laboratory (Towle, Mariash, and Oppenheimer,1980. 
      Changes in hepatic levels of messenger ribonucleic acid for malic enzyme during 
      induction by thyroid hormone or diet. Biochemistry. 19: 579-585.) show that ME 
      induction both by CHO and T3 is mediated by an increase in specific messenger RNA 
      for ME, the interaction of T3 and the dietary factor occurs at a pretanslational 
      level.
FAU - Mariash, C N
AU  - Mariash CN
FAU - Kaiser, F E
AU  - Kaiser FE
FAU - Schwartz, H L
AU  - Schwartz HL
FAU - Towle, H C
AU  - Towle HC
FAU - Oppenheimer, J H
AU  - Oppenheimer JH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Lipids)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.1.1.43 (Phosphogluconate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
SB  - IM
MH  - Adipose Tissue/enzymology
MH  - Animals
MH  - Dietary Carbohydrates/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Enzyme Induction/drug effects
MH  - Fatty Acid Synthases/biosynthesis
MH  - Glucosephosphate Dehydrogenase/biosynthesis
MH  - Lipids/*biosynthesis
MH  - Liver/drug effects/*enzymology
MH  - Malate Dehydrogenase/biosynthesis
MH  - Male
MH  - Phosphogluconate Dehydrogenase/biosynthesis
MH  - Rats
MH  - Time Factors
MH  - Triiodothyronine/*pharmacology
PMC - PMC371445
EDAT- 1980/05/01 00:00
MHDA- 1980/05/01 00:01
PMCR- 1980/05/01
CRDT- 1980/05/01 00:00
PHST- 1980/05/01 00:00 [pubmed]
PHST- 1980/05/01 00:01 [medline]
PHST- 1980/05/01 00:00 [entrez]
PHST- 1980/05/01 00:00 [pmc-release]
AID - 10.1172/JCI109766 [doi]
PST - ppublish
SO  - J Clin Invest. 1980 May;65(5):1126-34. doi: 10.1172/JCI109766.