PMID- 7387739
OWN - NLM
STAT- MEDLINE
DCOM- 19800828
LR  - 20190718
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 23
IP  - 6
DP  - 1980 Jun
TI  - Application of a radiometric ear assay for studies of adjuvant arthritis in rats.
PG  - 633-40
AB  - A radioisotopic method, originally developed for measuring the cellular response 
      in delayed hypersensitivity lesions in mice, has been evaluated in adjuvant 
      arthritic rats. Focal accumulation of 5-iodo-2'-deoxyuridine-125I (125IUdR) at a 
      site of antigen challenge (left pinna) was measured and expressed as increased 
      radioactivity in the challenged (left) over the unchallenged (right) ear (L/R ear 
      ratio). Immunologic specificity of the assay was established with anti-lymphocyte 
      globulin (ALG)-treated arthritic rats. ALG significantly inhibited the 125IUdR 
      L/R ear ratio; normal rabbit globulin had no effect on this parameter. A 
      significant negative correlation was observed between the 125IUdR ear ratios and 
      subjective arthritic scores in established adjuvant disease. Certain 
      characteristics of the 125IUdR radiometric ear assay in rats were established in 
      several types of experiments: disappearance of the isotope from blood, whole body 
      irradiation studies in turpentine-injected rats, and cyclophosphamide 
      pretreatment in a sheep erythrocyte antigenic system. The results of this study 
      support the utility of the 125IUdR ear assay to quantify cellular accumulation at 
      a site of antigen challenge in adjuvant arthritic rats and possibly other 
      antigenic systems in this species.
FAU - Gans, K R
AU  - Gans KR
FAU - Heyner, S
AU  - Heyner S
FAU - Orzechowski, R F
AU  - Orzechowski RF
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Iodine Radioisotopes)
RN  - LGP81V5245 (Idoxuridine)
SB  - IM
MH  - Animals
MH  - Arthritis/*diagnostic imaging
MH  - Arthritis, Experimental/*diagnostic imaging
MH  - Ear/immunology
MH  - Hypersensitivity, Delayed/diagnostic imaging
MH  - Idoxuridine
MH  - Immunity, Cellular/radiation effects
MH  - *Iodine Radioisotopes
MH  - Male
MH  - Radiometry/*methods
MH  - Radionuclide Imaging
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1980/06/01 00:00
MHDA- 1980/06/01 00:01
CRDT- 1980/06/01 00:00
PHST- 1980/06/01 00:00 [pubmed]
PHST- 1980/06/01 00:01 [medline]
PHST- 1980/06/01 00:00 [entrez]
AID - 10.1002/art.1780230604 [doi]
PST - ppublish
SO  - Arthritis Rheum. 1980 Jun;23(6):633-40. doi: 10.1002/art.1780230604.