PMID- 7464946
OWN - NLM
STAT- MEDLINE
DCOM- 19810424
LR  - 20190618
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 289
IP  - 5800
DP  - 1981 Feb 26
TI  - Reaction of 5-iodonaphthyl-1-nitrene with the IgE receptor on normal and tumour 
      mast cells.
PG  - 806-8
AB  - Mast cells, basophils and a tumour analogue--rat basophilic leukaemia (RBL) 
      cells--have a surface glycoprotein (R epsilon) which specifically binds monomeric 
      immunoglobulin E (IgE), and aggregation of R epsilon causes secretion. When 
      isolated from non-ionic detergent extracts of surface-labelled RBL cells by 
      IgE-specific immunoprecipitation R epsilon appears as a 50,000 (50 K) to 60,000 
      (60 K) molecular weight (MW) band on electrophoresis in polyacrylamide gels in 
      SDS (SDS-PAGE). Likewise, only a 50 component is observed when the polypeptide 
      that binds IgE is isolated by affinity chromatography in conditions which prevent 
      aggregation of the IgE, even when intrinsically labelled R epsilon is studied. To 
      determine how R epsilon is inserted into the plasma membrane, we reacted RBL 
      cells with the photolysable hydrophobic reagent 5-iodonaphthyl-1-azide (INA), 
      which preferentially labels the intramembranous segments of several intrinsic 
      membrane proteins. We report here that, surprisingly, the label was found, not on 
      the 50 K glycopeptide, but only on a 30 K component which other studies suggest 
      is a subunit of R epsilon (ref 9, 12).
FAU - Holowka, D
AU  - Holowka D
FAU - Gitler, C
AU  - Gitler C
FAU - Bercovici, T
AU  - Bercovici T
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Azides)
RN  - 0 (Glycoproteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Lipids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Naphthalenes)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 63785-44-4 (iodonaphthylazide)
SB  - IM
MH  - Animals
MH  - *Azides
MH  - Basophils/*metabolism
MH  - Glycoproteins/metabolism
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia, Experimental/*metabolism
MH  - Macromolecular Substances
MH  - Mast Cells/*metabolism
MH  - Membrane Lipids
MH  - Membrane Proteins
MH  - Molecular Weight
MH  - *Naphthalenes
MH  - Rats
MH  - Receptors, Immunologic/*metabolism
EDAT- 1981/02/26 00:00
MHDA- 1981/02/26 00:01
CRDT- 1981/02/26 00:00
PHST- 1981/02/26 00:00 [pubmed]
PHST- 1981/02/26 00:01 [medline]
PHST- 1981/02/26 00:00 [entrez]
AID - 10.1038/289806a0 [doi]
PST - ppublish
SO  - Nature. 1981 Feb 26;289(5800):806-8. doi: 10.1038/289806a0.