PMID- 7470442
OWN - NLM
STAT- MEDLINE
DCOM- 19810521
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 19
IP  - 26
DP  - 1980 Dec 23
TI  - Initiation of reovirus transcription by inosine 5'-triphosphate and properties of 
      7-methylinosine-capped, inosine-substituted messenger ribonucleic acids.
PG  - 5960-6
AB  - Inosine 5'-triphosphate (ITP) can be utilized in place of guanosine 
      5'-triphosphate (GTP) for both the initiation and the elongation steps of 
      reovirus transcription, resulting in the synthesis of mRNAs containing 
      5'-terminal m7IpppIm and internal pI. The apparent molecular weights of the 
      I-substituted products were altered as a consequence of the absence of G-C base 
      pairs and accompanying loss of ordered structure. The migration of I-substituted 
      RNAs in agarose gels and glycerol gradients was similar to glyoxal-treated 
      transcripts; i.e., it decreased 2-fold as compared to the corresponding untreated 
      G-containing mRNAs. 7-Methylinosine-capped (m7I-capped), I-substituted 
      transcripts readily attached to wheat germ 80S ribosomes. Unlike native 
      G-containing mRNAs, they also formed heavier complexes that sedimented faster 
      than 80S complexes even in the presence of the nonhydrolyzable ATP analogue 
      AMPP(NH)P and elongation inhibitor sparsomycin. I-substituted molecules that were 
      capped posttranscriptionally to form m7G-capped 5' ends yielded mostly 80S 
      monosomes, consistent with a strong influence of 5'-terminal structure on 
      initiation of translation. Under limited conditions of initiation, I-substituted 
      RNAs outcompeted G-containing transcripts for ribosome attachment. Although the 
      results are consistent with enhanced binding and freer movement of ribosomes on 
      unstructured templates, synthesis of acid-precipitable polypeptides in wheat germ 
      extract directed by I-substituted RNAs was 15-fold less than with G-containing 
      mRNAs.
FAU - Morgan, M A
AU  - Morgan MA
FAU - Shatkin, A J
AU  - Shatkin AJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Inosine Nucleotides)
RN  - 0 (RNA Cap Analogs)
RN  - 0 (RNA Caps)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Viral)
RN  - 132-06-9 (Inosine Triphosphate)
SB  - IM
MH  - Binding Sites
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Inosine Nucleotides/*metabolism
MH  - Inosine Triphosphate/*metabolism
MH  - Mammalian orthoreovirus 3/*metabolism
MH  - Molecular Weight
MH  - RNA Cap Analogs/*metabolism
MH  - RNA Caps/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - RNA, Ribosomal/metabolism
MH  - RNA, Viral/*metabolism
MH  - Reoviridae/*metabolism
MH  - *Transcription, Genetic
MH  - Triticum/metabolism
EDAT- 1980/12/23 00:00
MHDA- 1980/12/23 00:01
CRDT- 1980/12/23 00:00
PHST- 1980/12/23 00:00 [pubmed]
PHST- 1980/12/23 00:01 [medline]
PHST- 1980/12/23 00:00 [entrez]
AID - 10.1021/bi00567a003 [doi]
PST - ppublish
SO  - Biochemistry. 1980 Dec 23;19(26):5960-6. doi: 10.1021/bi00567a003.