PMID- 7472890 OWN - NLM STAT- MEDLINE DCOM- 19951130 LR - 20041117 IS - 0022-3395 (Print) IS - 0022-3395 (Linking) VI - 81 IP - 5 DP - 1995 Oct TI - Hammondia hammondi cysts in cell cultures. PG - 821-4 AB - In an attempt to obtain the continuous development of Hammondia hammondi in vitro, culture of the parasite was performed in 3 cell lines. Although organisms were present in culture fluids of feline kidney cells (CRFK) for as long as 3 mo, continuous culture was not possible. However, for the first time, cysts of H. hammondi were observed in cell culture from day 6 after inoculation of sporozoites. Ultrastructure of the H. hammondi cysts was similar to that observed for in vitro-obtained Toxoplasma gondii cysts. Feeding a cat with these in vitro-developed cysts resulted in oocyst shedding 5 days after ingestion. FAU - Riahi, H AU - Riahi H AD - Service de Parasitologie-Mycologie, CHU Dupuytren, Limoges, France. FAU - Darde, M L AU - Darde ML FAU - Bouteille, B AU - Bouteille B FAU - Leboutet, M J AU - Leboutet MJ FAU - Pestre-Alexandre, M AU - Pestre-Alexandre M LA - eng PT - Journal Article PL - United States TA - J Parasitol JT - The Journal of parasitology JID - 7803124 SB - IM MH - Animals MH - Cats MH - Cattle MH - Cell Line MH - Coccidia/*growth & development/ultrastructure MH - Fibroblasts MH - Humans MH - Kidney MH - Mice EDAT- 1995/10/01 00:00 MHDA- 1995/10/01 00:01 CRDT- 1995/10/01 00:00 PHST- 1995/10/01 00:00 [pubmed] PHST- 1995/10/01 00:01 [medline] PHST- 1995/10/01 00:00 [entrez] PST - ppublish SO - J Parasitol. 1995 Oct;81(5):821-4.