PMID- 7473044
OWN - NLM
STAT- MEDLINE
DCOM- 19951213
LR  - 20061115
IS  - 0914-5087 (Print)
IS  - 0914-5087 (Linking)
VI  - 26
IP  - 3
DP  - 1995 Sep
TI  - [Immunohistochemical analysis of adhesion molecules in directional coronary 
      atherectomy specimens].
PG  - 139-47
AB  - Chronic inflammatory cells are key components in the progression of 
      atherosclerotic plaques and restenosis after coronary angioplasty. Adhesion 
      molecules are fundamental in inflammatory processes. Therefore, the distributions 
      of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule 
      (VCAM) were investigated in directional coronary atherectomy specimens obtained 
      from 14 patients, in 6 with acute coronary syndromes (myocardial infarction and 
      unstable angina within 1 month), 6 with old myocardial infarction and 2 with 
      stable effort angina. There were eight primary lesions and six restenotic 
      lesions. Atherectomy tissue fragments were snap frozen and cut into 4 microns 
      thick cryostat sections for immunohistochemical staining by avidin-biotin complex 
      immunoperoxidase techniques using adhesion molecule specific monoclonal 
      antibodies BBIG-I1 (ICAM-1) and BBIG-V1 (VCAM). The cells of lesions were 
      characterized in sequential sections by macrophage marker KP1 (CD68), endothelial 
      marker JC/70A (CD31), and smooth muscle cell marker 1A4 (alpha-smooth muscle 
      actin). Four restenotic lesions that had undergone a prior balloon angioplasty 
      within a few months consisted of intimal proliferation and the other lesions were 
      atherosclerotic plaque. Macrophage-rich areas were seen in the lesions from acute 
      coronary syndromes and/or early restenotic lesions. Expression of ICAM-1 or VCAM 
      was strongly associated with macrophage-rich areas, but VCAM staining was weaker 
      than ICAM-1 except in one restenotic lesion. Macrophages that express ICAM-1 
      and/or VCAM may be important in the unstable plaques and restenotic lesions 
      related to disease activity of ischemic heart disease.
FAU - Sakurai, S
AU  - Sakurai S
AD  - Third Department of Internal Medicine, Shinshu University School of Medicine, 
      Matsumoto.
FAU - Inoue, A
AU  - Inoue A
FAU - Ohwa, M
AU  - Ohwa M
FAU - Koh, C S
AU  - Koh CS
FAU - Ohkubo, K
AU  - Ohkubo K
FAU - Hikita, H
AU  - Hikita H
FAU - Kohno, H
AU  - Kohno H
FAU - Yanagisawa, N
AU  - Yanagisawa N
LA  - jpn
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
PL  - Netherlands
TA  - J Cardiol
JT  - Journal of cardiology
JID - 8804703
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Aged
MH  - *Atherectomy, Coronary
MH  - Coronary Disease/*metabolism/surgery
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Intercellular Adhesion Molecule-1/*analysis
MH  - Macrophage Activation
MH  - Macrophages/metabolism
MH  - Male
MH  - Middle Aged
MH  - Vascular Cell Adhesion Molecule-1/*analysis
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
PST - ppublish
SO  - J Cardiol. 1995 Sep;26(3):139-47.