PMID- 7475268
OWN - NLM
STAT- MEDLINE
DCOM- 19951218
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 9
IP  - 11
DP  - 1995 Nov
TI  - Cytogenetic clonality analysis in myelodysplastic syndrome: monosomy 7 can be 
      demonstrated in the myeloid and in the lymphoid lineage.
PG  - 1818-21
AB  - Bone marrow and blood from three patients with myelodysplastic syndrome (MDS) and 
      monosomy 7 were studied for cell lineage involvement of the chromosomal 
      abnormality. Cytogenetic involvement of the myeloid and erythroid cell lineages 
      in MDS with monosomy 7 has been shown before. Lymphoid subpopulations have also 
      been investigated but generally with negative results. A combined technique of 
      May-Grunwald-Giemsa (MGG) for cell cytology and interphase fluorescence in situ 
      hybridization (FISH) using a chromosome 7 specific DNA probe was applied. 
      Further, immunophenotype and genotype of the cells were simultaneously examined 
      with alkaline phosphatase anti-alkaline phosphatase (APAAP) immunostaining and 
      FISH. The monosomy 7 was found in the blasts and in all or in subpopulations of 
      myeloid and erythroid cells. T cells (CD3+, CD5+) did not appear to be involved. 
      B cells (CD19+, CD22+) showed a normal distribution of FISH spots in two 
      patients. In one patient however the loss of a chromosome 7 was found in 
      approximately 70% of the cells positive for B cell markers including CD79a. The 
      results of this study show that in some cases MDS is a disease arising in a 
      progenitor cell with repopulative abilities restricted to myelopoiesis and 
      erythropoiesis. In other cases, the pluripotent progenitor cells in MDS may show 
      the capacities to differentiate into B lineage lymphoid cells, as well as 
      suggesting that in those instances MDS represents a condition of more primitive 
      transformed hematopoietic ancestor cells.
FAU - van Lom, K
AU  - van Lom K
AD  - Department of Hematology, University Hospital, Rotterdam, The Netherlands.
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Smit, E
AU  - Smit E
FAU - Hahlen, K
AU  - Hahlen K
FAU - Groeneveld, K
AU  - Groeneveld K
FAU - Lowenberg, B
AU  - Lowenberg B
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - Child
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Disorders
MH  - *Chromosomes, Human, Pair 7
MH  - Clone Cells
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Monosomy/*genetics
MH  - Myelodysplastic Syndromes/*genetics/pathology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1995 Nov;9(11):1818-21.